TY - JOUR
T1 - Wnt-5a expression in the rat neuronal progenitor cell line ST14A
AU - Peters, Sabine
AU - Mix, Eilhard
AU - Bauer, Peter
AU - Weinelt, Sabine
AU - Schubert, Beke
AU - Knoblich, Rupert
AU - Böttcher, Tobias
AU - Strauss, Ulf
AU - Pahnke, Jens
AU - Cattaneo, Elena
AU - Wree, Andreas
AU - Rolfs, Arndt
N1 - Funding Information:
Acknowledgements We thank Dr. Roel Nusse, Howard Hughes Medical Institute, Department of Developmental Biology, Beckman Center, Stanford Medical School, and Dr. Robert Jaster, Department of Gastroenterology, University of Rostock, for helpful advice on the design of experiments and for critical comments on the manuscript. This work was supported by grants of the Federal Ministry of Education and Research (BMBF) (BEO31– 01K003135201–031U214A) and of the Ministry of Education, Science and Culture of Mecklenburg-Vorpommern as well as by an intramural grant (FORUN application). We thank Dr. Lary C. Walker (Atlanta) for helpful comments on the manuscript as well as Waltraut Gericke for technical support.
PY - 2004/9
Y1 - 2004/9
N2 - Transplantation of cells derived from embryonic stem cells is currently under investigation as a promising strategy to restore functional deficits in neurodegenerative diseases, e.g. Parkinson's disease. To generate cells suitable for transplantation, a neuronal progenitor cell line (ST14A) was derived from embryonic day 14 rat striatum by stable retroviral transfection of the temperature-sensitive SV40 large T antigen and genetically modified by transfection with constructs of the neurotrophic factors ciliary neurotrophic factor (CNTF) [CNTF-ST14A] and glial cell line-derived neurotrophic factor (GDNT) [GDNF-ST14A], respectively. In order to investigate the capacity of these cells to regulate neuronal growth and physiological differentiation, e.g. remodeling of axons and synaptogenesis, we analyzed the expression of molecules which control the cell fate during embryonic development. For the first time, we found endogenous Wnt-5a, a regulator molecule that can induce dopaminergic phenotype, by RT-PCR, Western blot and flow cytometry in the neuronal progenitor cell line ST14A and its derivatives CNTF-ST14A and GDNF-ST14A. The protein was transiently upregulated at the differentiation-inducing non-permissive temperature of 39°C and it was also secreted into the culture medium. Our findings are based on in vitro investigation of artificially immortalized cell lines. However, they raise the possibility that neuronal progenitor cells that might be used to treat neurodegenerative diseases express Wnt-5a, thus promoting their potential for dopaminergic differentiation.
AB - Transplantation of cells derived from embryonic stem cells is currently under investigation as a promising strategy to restore functional deficits in neurodegenerative diseases, e.g. Parkinson's disease. To generate cells suitable for transplantation, a neuronal progenitor cell line (ST14A) was derived from embryonic day 14 rat striatum by stable retroviral transfection of the temperature-sensitive SV40 large T antigen and genetically modified by transfection with constructs of the neurotrophic factors ciliary neurotrophic factor (CNTF) [CNTF-ST14A] and glial cell line-derived neurotrophic factor (GDNT) [GDNF-ST14A], respectively. In order to investigate the capacity of these cells to regulate neuronal growth and physiological differentiation, e.g. remodeling of axons and synaptogenesis, we analyzed the expression of molecules which control the cell fate during embryonic development. For the first time, we found endogenous Wnt-5a, a regulator molecule that can induce dopaminergic phenotype, by RT-PCR, Western blot and flow cytometry in the neuronal progenitor cell line ST14A and its derivatives CNTF-ST14A and GDNF-ST14A. The protein was transiently upregulated at the differentiation-inducing non-permissive temperature of 39°C and it was also secreted into the culture medium. Our findings are based on in vitro investigation of artificially immortalized cell lines. However, they raise the possibility that neuronal progenitor cells that might be used to treat neurodegenerative diseases express Wnt-5a, thus promoting their potential for dopaminergic differentiation.
UR - http://www.scopus.com/inward/record.url?scp=2942756476&partnerID=8YFLogxK
U2 - 10.1007/s00221-004-1887-0
DO - 10.1007/s00221-004-1887-0
M3 - Journal articles
C2 - 15309358
AN - SCOPUS:2942756476
SN - 0014-4819
VL - 158
SP - 189
EP - 195
JO - Experimental Brain Research
JF - Experimental Brain Research
IS - 2
ER -
