Whole-genome sequencing disproves two suspected transmission events of blaNDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients

K. Kocer; S. Boutin; K. Probst; K. Heeg; D. Nurjadi

doi:10.1016/j.jhin.2020.07.006

Whole-genome sequencing disproves two suspected transmission events of bla_NDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients

K. Kocer^*, S. Boutin, K. Probst, K. Heeg, D. Nurjadi

^*Corresponding author for this work

Ruprecht-Karls Universität Heidelberg

Abstract

New Delhi metallo-β-lactamase (bla_NDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of bla_NDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different bla_NDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks.

Original language	English
Journal	Journal of Hospital Infection
Volume	106
Issue number	2
Pages (from-to)	372-375
Number of pages	4
ISSN	0195-6701
DOIs	https://doi.org/10.1016/j.jhin.2020.07.006
Publication status	Published - 10.2020

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10.1016/j.jhin.2020.07.006

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title = "Whole-genome sequencing disproves two suspected transmission events of blaNDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients",

abstract = "New Delhi metallo-β-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of blaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different blaNDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks.",

author = "K. Kocer and S. Boutin and K. Probst and K. Heeg and D. Nurjadi",

note = "Publisher Copyright: {\textcopyright} 2020 The Healthcare Infection Society",

year = "2020",

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language = "English",

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T1 - Whole-genome sequencing disproves two suspected transmission events of blaNDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients

AU - Kocer, K.

AU - Boutin, S.

AU - Probst, K.

AU - Heeg, K.

AU - Nurjadi, D.

PY - 2020/10

Y1 - 2020/10

N2 - New Delhi metallo-β-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of blaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different blaNDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks.

AB - New Delhi metallo-β-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of blaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different blaNDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks.

UR - https://www.scopus.com/pages/publications/85090413701

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DO - 10.1016/j.jhin.2020.07.006

M3 - Journal articles

C2 - 32652213

AN - SCOPUS:85090413701

SN - 0195-6701

VL - 106

SP - 372

EP - 375

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

IS - 2

ER -

Whole-genome sequencing disproves two suspected transmission events of bla_NDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients

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