Abstract
We have compared the efficiency of four methods to remove 2,3-diphosphoglycerate (DPG) from hemoglobin (Hb), comprising dialysis, repeated ultrafiltration, gel filtration, and ion-exchange chromatography. All the methods eventually yielded hemoglobin solutions with a ratio of <0.002 mol of DPG/mol of Hb4 and identical oxygen-binding properties but differed with respect to duration and versatility. Considering these factors, we found the combination of an ion-retardation resin and a mixed-bed resin to be most satisfactory.
| Original language | English |
|---|---|
| Journal | Analytical Biochemistry |
| Volume | 75 |
| Issue number | 2 |
| Pages (from-to) | 382-388 |
| Number of pages | 7 |
| ISSN | 0003-2697 |
| DOIs | |
| Publication status | Published - 10.1976 |
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This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Research Areas and Centers
- Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)
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