What is the best method to remove 2,3-diphosphoglycerate from hemoglobin?

Wolfgang Jelkmann*, Christian Bauer

*Corresponding author for this work
59 Citations (Scopus)


We have compared the efficiency of four methods to remove 2,3-diphosphoglycerate (DPG) from hemoglobin (Hb), comprising dialysis, repeated ultrafiltration, gel filtration, and ion-exchange chromatography. All the methods eventually yielded hemoglobin solutions with a ratio of <0.002 mol of DPG/mol of Hb4 and identical oxygen-binding properties but differed with respect to duration and versatility. Considering these factors, we found the combination of an ion-retardation resin and a mixed-bed resin to be most satisfactory.

Original languageEnglish
JournalAnalytical Biochemistry
Issue number2
Pages (from-to)382-388
Number of pages7
Publication statusPublished - 10.1976

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)


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