Abstract
Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Semiconductor diode seeded fiber lasers have proven to be especially robust, low cost and easy to use. However, their wavelength tuning range is often limited, so only a limited number of fluorophores can be accessed. Therefore, different approaches have been proposed to extend the spectral coverage of these lasers. Recently, we showed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS) can be harnessed to red-shift high power pulses from 1064 nm to a narrowband output at 1122 nm and 1186 nm and therefore extend the number of accessible fluorophores. In this contribution, we show the applicability of all three wavelengths for multi-photon microscopy and analyze the performance.
| Original language | English |
|---|---|
| Article number | #345779 |
| Journal | Biomedical Optics Express |
| Volume | 9 |
| Issue number | 12 |
| Pages (from-to) | 6273-6282 |
| Number of pages | 10 |
| DOIs | |
| Publication status | Published - 01.12.2018 |
Funding
This Research was funded by the European Union project ENCOMOLE-2i (Horizon 2020, ERC CoG no. 646669); the German Research Foundation (DFG project HU1006/6 and EXC 306/2); the European Union within Interreg Deutschland-Danmark from the European Regional Development Fund in the project CELLTOM; the German Federal Ministry of Education and Research (BMBF no. 13GW0227B: “Neuro-OCT”); the Schleswig-Holstein ExcellenceChair program within the Cluster of Excellence Inflammation at Interfaces jointly funded by the German Research Foundation (DFG) and the state of Schleswig-Holstein.
Research Areas and Centers
- Academic Focus: Biomedical Engineering
