Wavelength agile multi-photon microscopy with a fiber amplified diode laser

Matthias Eibl, Daniel Weng, Hubertus Hakert, Jan Philip Kolb, Tom Pfeiffer, Jennifer E. Hundt, Robert Huber, Sebastian Karpf*

*Corresponding author for this work


Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Semiconductor diode seeded fiber lasers have proven to be especially robust, low cost and easy to use. However, their wavelength tuning range is often limited, so only a limited number of fluorophores can be accessed. Therefore, different approaches have been proposed to extend the spectral coverage of these lasers. Recently, we showed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS) can be harnessed to red-shift high power pulses from 1064 nm to a narrowband output at 1122 nm and 1186 nm and therefore extend the number of accessible fluorophores. In this contribution, we show the applicability of all three wavelengths for multi-photon microscopy and analyze the performance.

Original languageEnglish
Article number#345779
JournalBiomedical Optics Express
Issue number12
Pages (from-to)6273-6282
Number of pages10
Publication statusPublished - 01.12.2018

Research Areas and Centers

  • Academic Focus: Biomedical Engineering


Dive into the research topics of 'Wavelength agile multi-photon microscopy with a fiber amplified diode laser'. Together they form a unique fingerprint.

Cite this