Abstract
Background: Vitamin D exhibits multiple antiproliferative and pro-differentiating actions. Prostaglandin-(PG)E2 is a tumor-promoting tissue hormone anabolized by cyclooxygenase-2 (COX-2). Recently, a link between the PG and vitamin D metabolism was reported. Materials and Methods: The influence of calcitriol and celecoxib on the proliferation of ovarian cancer cell lines was measured and the impact of calcitriol on the protein and mRNA expression of COX-2 was quantified by western blot and qRT-PCR, respectively. Results: After COX-2 induction with interleukin (IL)-1β, 10 μM celecoxib did not significantly inhibit the proliferation of OVCAR-3 cells, whereas calcitriol showed such an effect; however, the combination of the two substances had an additive influence. After induction by IL-1β, calcitriol inhibited the COX-2 protein, as well as its mRNA expression significantly in OVCAR-3 and SKOV-3 cells. Conclusion: These data suggest a correlation between PG and vitamin D metabolism in their anti-tumorigenic activity in ovarian carcinomas.
Original language | English |
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Journal | Anticancer Research |
Volume | 35 |
Issue number | 2 |
Pages (from-to) | 1197-1203 |
Number of pages | 7 |
ISSN | 0250-7005 |
Publication status | Published - 01.01.2015 |
Research Areas and Centers
- Research Area: Luebeck Integrated Oncology Network (LION)