Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

S. Kügler; A. Grünweller; C. Probst; M. Klinger; P. K. Müller; C. Kruse

doi:10.1016/0014-5793(96)00204-9

Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

S. Kügler, A. Grünweller, C. Probst, M. Klinger, P. K. Müller, C. Kruse^*

^*Corresponding author for this work

37 Citations (Scopus)

Abstract

Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH₂-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.

Original language	English
Journal	FEBS Letters
Volume	382
Issue number	3
Pages (from-to)	330-334
Number of pages	5
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(96)00204-9
Publication status	Published - 18.03.1996

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1016/0014-5793(96)00204-9

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abstract = "Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.",

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T1 - Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

AU - Kügler, S.

AU - Grünweller, A.

AU - Probst, C.

AU - Klinger, M.

AU - Müller, P. K.

AU - Kruse, C.

PY - 1996/3/18

Y1 - 1996/3/18

N2 - Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.

AB - Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.

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U2 - 10.1016/0014-5793(96)00204-9

DO - 10.1016/0014-5793(96)00204-9

M3 - Journal articles

C2 - 8605996

AN - SCOPUS:0343113370

SN - 0014-5793

VL - 382

SP - 330

EP - 334

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

Abstract

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UN SDGs

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