TY - JOUR
T1 - Ultrafast Spectral Tuning of a Fiber Laser for Time-Encoded Multiplex Coherent Raman Scattering Microscopy
AU - Gottschall, Thomas
AU - Meyer-Zedler, Tobias
AU - Eibl, Matthias
AU - Pfeiffer, Tom
AU - Hakert, Hubertus
AU - Schmitt, Michael
AU - Huber, Robert
AU - Tünnermann, Andreas
AU - Limpert, Jens
AU - Popp, Juergen
N1 - Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/3/23
Y1 - 2023/3/23
N2 - Coherent Raman scattering microscopy utilizing bioorthogonal tagging approaches like isotope or alkyne labeling allows for a targeted monitoring of spatial distribution and dynamics of small molecules of interest in cells, tissues, and other complex biological matrices. To fully exploit this approach in terms of real-time monitoring of several Raman tags, e.g., to study drug uptake dynamics, extremely fast tunable lasers are needed. Here, we present a laser concept without moving parts and fully electronically controlled for the quasi-simultaneous acquisition of coherent anti-Stokes Raman scattering images at multiple Raman resonances. The laser concept is based on the combination of a low noise and spectrally narrow Fourier domain mode-locked laser seeding a compact four wave mixing-based high-power fiber-based optical parametric amplifier.
AB - Coherent Raman scattering microscopy utilizing bioorthogonal tagging approaches like isotope or alkyne labeling allows for a targeted monitoring of spatial distribution and dynamics of small molecules of interest in cells, tissues, and other complex biological matrices. To fully exploit this approach in terms of real-time monitoring of several Raman tags, e.g., to study drug uptake dynamics, extremely fast tunable lasers are needed. Here, we present a laser concept without moving parts and fully electronically controlled for the quasi-simultaneous acquisition of coherent anti-Stokes Raman scattering images at multiple Raman resonances. The laser concept is based on the combination of a low noise and spectrally narrow Fourier domain mode-locked laser seeding a compact four wave mixing-based high-power fiber-based optical parametric amplifier.
UR - https://doi.org/10.1021/acs.jpcb.2c09115
UR - http://www.scopus.com/inward/record.url?scp=85150437028&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/e0cff44e-8bc3-3ed9-88d6-1c0f9c393dd8/
U2 - 10.1021/acs.jpcb.2c09115
DO - 10.1021/acs.jpcb.2c09115
M3 - Journal articles
VL - 127
SP - 2375
EP - 2380
JO - The Journal of Physical Chemistry B
JF - The Journal of Physical Chemistry B
IS - 11
ER -