TY - JOUR
T1 - Two common polymorphisms in the peroxisome proliferator-activated receptor γ gene may improve fertilization in IVF
AU - Sahmani, Mehdi
AU - Sakhinia, Ebrahim
AU - Farzadi, Laya
AU - Najafipour, Reza
AU - Darabi, Masoud
AU - Mehdizadeh, Amir
AU - Shahnazi, Vahideh
AU - Shaaker, Maghsod
AU - Noori, Mohammad
N1 - Funding Information:
This study was partially supported by grants from the Drug Applied Research Centre (Grant No. 14/88 ), Tabriz University of Medical Sciences.
PY - 2011/9
Y1 - 2011/9
N2 - Genetic factors play an important role in women's fertility and embryonic development and may also contribute to the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of His447His and Pro12Ala peroxisome proliferator-activated receptor γ (PPARγ) gene polymorphisms on oocytes and fertilization in women undergoing IVF. Follicular fluid and blood samples were obtained from 98 IVF patients referred to Tabriz Alzahra Hospital. Samples were analysed for fatty acid content by gas-liquid chromatography and for polymorphisms of the PPARγ gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. Multiple regression analyses were used to test the independence of associations between the number of mature and fertilized oocytes as outcome variables and the polymorphisms of PPARγ gene. For both polymorphisms, fertilization ratio was significantly (P < 0.05) higher in carriers of the rare alleles than homozygous wild-type genotypes. The associations of His447His (P = 0.003) and Pro12Ala (P = 0.015) polymorphisms remained statistically significant in the multiple regression analyses. This study suggests that the two common gene polymorphisms of PPARγ may improve fertilization in vitro and, thus possibly, female fertility.
AB - Genetic factors play an important role in women's fertility and embryonic development and may also contribute to the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of His447His and Pro12Ala peroxisome proliferator-activated receptor γ (PPARγ) gene polymorphisms on oocytes and fertilization in women undergoing IVF. Follicular fluid and blood samples were obtained from 98 IVF patients referred to Tabriz Alzahra Hospital. Samples were analysed for fatty acid content by gas-liquid chromatography and for polymorphisms of the PPARγ gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. Multiple regression analyses were used to test the independence of associations between the number of mature and fertilized oocytes as outcome variables and the polymorphisms of PPARγ gene. For both polymorphisms, fertilization ratio was significantly (P < 0.05) higher in carriers of the rare alleles than homozygous wild-type genotypes. The associations of His447His (P = 0.003) and Pro12Ala (P = 0.015) polymorphisms remained statistically significant in the multiple regression analyses. This study suggests that the two common gene polymorphisms of PPARγ may improve fertilization in vitro and, thus possibly, female fertility.
UR - http://www.scopus.com/inward/record.url?scp=80052410877&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2011.05.005
DO - 10.1016/j.rbmo.2011.05.005
M3 - Journal articles
C2 - 21764381
AN - SCOPUS:80052410877
SN - 1472-6483
VL - 23
SP - 355
EP - 360
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 3
ER -
