Abstract
Tumor necrosis factor α (TNFα) is thought to contribute to the blunted erythropoietin (Epo) production in inflammatory diseases. The present study was carried out to find out as to whether the 55 kD (TNF-RI) or the 75 kD (TNF-RH) receptor is responsible for the TNFα-induced inhibition of hepatic Epo synthesis. When the effects of two receptor-specific mutants were compared, only the TNF-RI-specific isoform proved to suppress the formation of immunoreactive Epo in the human hepatoma cell lines HepG2 and Hep3B, similar to the effect of wild-type TNFα. Anti-TNFα antibody restored Epo production in TNFα- or TNF-RI mutant-treated cultures. By gel shift assay NF-κB binding to DNA was demonstrated following the addition of TNFα or TNF-RI-specific mutant to HepG2 cells, while the TNF-RII-specific mutant was ineffective. Finally, immunoreactive TNF-RI, but not TNF-RII, fragments were measurable in cell culture supernatants. Taken together, these results suggest that the inhibition of hepatic Epo production by TNFα is mediated by TNF-RI signaling.
| Original language | English |
|---|---|
| Journal | Experimental Hematology |
| Volume | 27 |
| Issue number | 2 |
| Pages (from-to) | 224-228 |
| Number of pages | 5 |
| ISSN | 0301-472X |
| DOIs | |
| Publication status | Published - 02.1999 |
Funding
The authors are indebted to Dr. W. Lesslauer and Dr. H. Loetscher (F. Hoffmann-La Roche Ltd., Basel, Switzerland) for the supply of TNF receptor-specific mutants. The study was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 367-C8).
Research Areas and Centers
- Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)
