TY - JOUR
T1 - Tumor budding and lymphovascular invasion as prognostic factors in p16-positive oropharyngeal squamous cell carcinomas
T2 - Molecular Diagnostics
AU - Stögbauer, Fabian
AU - Wirth, Markus
AU - Lauterbach, Maren
AU - Wollenberg, Barbara
AU - Schmidl, Benedikt
AU - Hoch, Cosima C.
AU - Ourailidis, Iordanis
AU - Hess, Jochen
AU - Eckstein, Markus
AU - Hartmann, Arndt
AU - Iro, Heinrich
AU - Gostian, Antoniu Oreste
AU - Balk, Matthias
AU - Jesinghaus, Moritz
AU - Ribbat-Idel, Julika
AU - Sailer, Verena Wilbeth
AU - Perner, Sven
AU - Bruchhage, Karl Ludwig
AU - Hoffmann, Markus
AU - Lükewille, Lukas
AU - Stuhlmann-Laeisz, Christiane Maria
AU - Röcken, Christoph
AU - Mogler, Carolin
AU - Budczies, Jan
AU - Boxberg, Melanie
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2025/2/10
Y1 - 2025/2/10
N2 - Background: We aimed to validate the prognostic significance of tumor budding (TB) in p16-positive oropharyngeal squamous cell carcinomas (OPSCC). Methods: We analyzed digitized H&E-stained slides from a multicenter cohort of five large university centers consisting of n = 275 cases of p16-positive OPSCC. We evaluated TB along with other histological parameters (morphology, tumor-stroma-ratio, lymphovascular invasion (LVI), perineural invasion) and calculated survival outcomes using both univariate and multivariate analyses. Results: TB was identified as an independent prognostic parameter, with TB-high cases showing inferior outcomes in univariate (HR: 3.08, 95%-CI: 1.71–5.54) and multivariate analyses (HR: 4.03, 95%-CI: 1.65–9.83). Similarly, LVI remained an independent prognostic factor (HR: 3.00, 95%-CI: 1.22–7.38). A combined classification including TB and LVI stratified cases into low-, intermediate- and high-risk categories. We could not detect correlations between TB and the number of lymph node metastases or between TB and an extracapsular extension of lymph node metastases. Conclusions: In addition to LVI, we could identify TB as an independent prognostic factor in p16-positive OPSCC in this multicenter study cohort. Thus, evaluating TB along with LVI in a combined scheme for prognostication might help to establish a more personalized treatment regimen for patients with p16-positive OPSCC.
AB - Background: We aimed to validate the prognostic significance of tumor budding (TB) in p16-positive oropharyngeal squamous cell carcinomas (OPSCC). Methods: We analyzed digitized H&E-stained slides from a multicenter cohort of five large university centers consisting of n = 275 cases of p16-positive OPSCC. We evaluated TB along with other histological parameters (morphology, tumor-stroma-ratio, lymphovascular invasion (LVI), perineural invasion) and calculated survival outcomes using both univariate and multivariate analyses. Results: TB was identified as an independent prognostic parameter, with TB-high cases showing inferior outcomes in univariate (HR: 3.08, 95%-CI: 1.71–5.54) and multivariate analyses (HR: 4.03, 95%-CI: 1.65–9.83). Similarly, LVI remained an independent prognostic factor (HR: 3.00, 95%-CI: 1.22–7.38). A combined classification including TB and LVI stratified cases into low-, intermediate- and high-risk categories. We could not detect correlations between TB and the number of lymph node metastases or between TB and an extracapsular extension of lymph node metastases. Conclusions: In addition to LVI, we could identify TB as an independent prognostic factor in p16-positive OPSCC in this multicenter study cohort. Thus, evaluating TB along with LVI in a combined scheme for prognostication might help to establish a more personalized treatment regimen for patients with p16-positive OPSCC.
UR - http://www.scopus.com/inward/record.url?scp=85210552318&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/ef1f1a42-b529-3e14-826f-226a016db49b/
U2 - 10.1038/s41416-024-02912-3
DO - 10.1038/s41416-024-02912-3
M3 - Journal articles
C2 - 39613843
AN - SCOPUS:85210552318
SN - 0007-0920
VL - 132
SP - 212
EP - 221
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 2
M1 - 524928
ER -
