TY - JOUR
T1 - Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum
AU - Mallagaray, Alvaro
AU - Rudolph, Lorena
AU - Lindloge, Melissa
AU - Mölbitz, Jarne
AU - Thomsen, Henrik
AU - Schmelter, Franziska
AU - Alhabash, Mohamad Ward
AU - Abdullah, Mohammed R.
AU - Saraei, Roza
AU - Ehlers, Marc
AU - Graf, Tobias
AU - Sina, Christian
AU - Petersmann, Astrid
AU - Nauck, Matthias
AU - Günther, Ulrich L.
N1 - Publisher Copyright:
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
PY - 2023/8/28
Y1 - 2023/8/28
N2 - Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N-glycans, respectively. Diffusion-edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p-value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo-metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID-19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls.
AB - Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N-glycans, respectively. Diffusion-edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p-value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo-metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID-19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls.
UR - http://www.scopus.com/inward/record.url?scp=85164939732&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/db6ef60d-cc22-3ef5-add3-fc56fee2bf2b/
U2 - 10.1002/anie.202306154
DO - 10.1002/anie.202306154
M3 - Journal articles
C2 - 37341676
AN - SCOPUS:85164939732
SN - 1433-7851
VL - 62
SP - e202306154
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 35
M1 - e202306154
ER -
