TNFA deletion alters apoptosis as well as caspase 3 and 4 expression during otitis media

Joerg Ebmeyer, Anke Leichtle, Michelle Hernandez, Umay Ebmeyer, Jacob Husseman, Kwang Pak, Holger Sudhoff, David Broide, Stephen I. Wasserman*, Allen F. Ryan

*Corresponding author for this work
18 Citations (Scopus)

Abstract

Background: Tumor necrosis factor (TNFA) is the canonical member of the TNF superfamily, which plays a major role in both inflammation and apoptosis. To evaluate the role of TNFs in otitis media (OM), the most common disease of childhood, we evaluated middle ear (ME) expression of genes encoding the TNF and TNF receptor superfamilies during bacterial OM in the mouse, characterized OM in TNFA-deficient mice, and assessed apoptosis during OM in normal versus TNF-deficient MEs.Results: TNFs and TNF receptors were broadly regulated during OM, with TNFA showing the highest level of up-regulation. TNF deficient mice exhibited mucosal hyperplasia even in the absence of infection and exuberant growth of the mucosa during OM, including the formation of mucosal polyps. Mucosal recovery during OM was also delayed, in parallel with a delay in mucosal apoptosis and reduced caspase gene expression.Conclusions: The TNF and TNF receptor superfamilies mediate both inflammation and apoptosis during OM. TNF appears to be critical for the maintenance of mucosal architecture in both the normal and infected ME, since excessive accumulation of mucosal tissue is seen in TNFA-/- MEs both before and after bacterial inoculation of the ME. TNFA is also required for appropriate regulation of caspase genes.

Original languageEnglish
Article number12
JournalBMC Immunology
Volume12
ISSN1471-2172
DOIs
Publication statusPublished - 26.01.2011

Fingerprint

Dive into the research topics of 'TNFA deletion alters apoptosis as well as caspase 3 and 4 expression during otitis media'. Together they form a unique fingerprint.

Cite this