TY - JOUR
T1 - Tissue fatty acid composition and secretory phospholipase-A2 activity in oral squamous cell carcinoma
AU - Askari, M.
AU - Darabi, M.
AU - Zare Mahmudabadi, R.
AU - Oboodiat, M.
AU - Fayezi, S.
AU - Mostakhdemin Hosseini, Z.
AU - Pirzadeh, A.
N1 - Funding Information:
This study was supported by the Cancer Research Center, Tehran University of Medical Sciences. A part of the study was performed as a DMD thesis. The authors acknowledge the Dental and Periodontal Research Center at Tabriz University of Medical Sciences. The authors would like to thank Masoud Darabi, PhD for reading the manuscript and providing helpful suggestions.
Publisher Copyright:
© 2014, Federación de Sociedades Españolas de Oncología (FESEO).
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Purpose: Oral squamous cell carcinoma (OSCC) is a remarkable health problem worldwide, but its pathogenesis remains unknown. The aim of this study was to compare fat composition and secretory phospholipase-A2 (sPLA2) activity between the malignant and adjacent normal squamous tissues in patients with OSCC. Methods: Paired samples of malignant squamous and adjacent normal-appearing tissues were collected from 27 patients with OSCC. The fatty acid composition in the obtained tissues was determined by gas liquid chromatography. Tissue enzyme activities of sPLA2 were measured using the standard assay with Diheptanoyl Thio-Phosphatidylcholine as substrate. Results: In the OSCC tissue, the level of stearic acid (18:0) and activity of sPLA2 were higher (P < 0.001), and the levels of oleic acid (18:1n-9) and linoleic acid (18:2n-6) were lower than that in the adjacent normal-appearing squamous tissue (P < 0.001). The activity of sPLA2 in OSCC was strongly negatively correlated with the amount of 18:2n-6 (r = −0.41, P < 0.001). Negative significant associations were observed between the OSCC invasion and tissue levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHE). Conclusion: The changes in the fatty acid composition and sPLA2 activity may be regarded as indicators of altered lipid metabolism occurring in vivo during squamous cell carcinogenesis.
AB - Purpose: Oral squamous cell carcinoma (OSCC) is a remarkable health problem worldwide, but its pathogenesis remains unknown. The aim of this study was to compare fat composition and secretory phospholipase-A2 (sPLA2) activity between the malignant and adjacent normal squamous tissues in patients with OSCC. Methods: Paired samples of malignant squamous and adjacent normal-appearing tissues were collected from 27 patients with OSCC. The fatty acid composition in the obtained tissues was determined by gas liquid chromatography. Tissue enzyme activities of sPLA2 were measured using the standard assay with Diheptanoyl Thio-Phosphatidylcholine as substrate. Results: In the OSCC tissue, the level of stearic acid (18:0) and activity of sPLA2 were higher (P < 0.001), and the levels of oleic acid (18:1n-9) and linoleic acid (18:2n-6) were lower than that in the adjacent normal-appearing squamous tissue (P < 0.001). The activity of sPLA2 in OSCC was strongly negatively correlated with the amount of 18:2n-6 (r = −0.41, P < 0.001). Negative significant associations were observed between the OSCC invasion and tissue levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHE). Conclusion: The changes in the fatty acid composition and sPLA2 activity may be regarded as indicators of altered lipid metabolism occurring in vivo during squamous cell carcinogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84928333306&partnerID=8YFLogxK
U2 - 10.1007/s12094-014-1242-2
DO - 10.1007/s12094-014-1242-2
M3 - Journal articles
C2 - 25351172
AN - SCOPUS:84928333306
SN - 1699-048X
VL - 17
SP - 378
EP - 383
JO - Clinical and Translational Oncology
JF - Clinical and Translational Oncology
IS - 5
ER -
