Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm−1

Hubertus Hakert; Matthias Eibl; Marie Tillich; Ralph Pries; Gereon Hüttmann; Ralf Brinkmann; Barbara Wollenberg; Karl Ludwig Bruchhage; Sebastian Karpf; Robert Huber

doi:10.1364/OL.424726

Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm⁻¹

Hubertus Hakert, Matthias Eibl, Marie Tillich, Ralph Pries, Gereon Hüttmann, Ralf Brinkmann, Barbara Wollenberg, Karl Ludwig Bruchhage, Sebastian Karpf, Robert Huber^*

^*Corresponding author for this work

8 Citations (Scopus)

Abstract

Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500−1800 cm⁻¹. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.

Original language	English
Journal	Optics Letters
Volume	46
Issue number	14
Pages (from-to)	3456-3459
Number of pages	4
ISSN	0146-9592
DOIs	https://doi.org/10.1364/OL.424726
Publication status	Published - 15.07.2021

Funding

Funding. European Regional Development Fund (CELLTOM); State of Schleswig Holstein (SH Chair); Bundesministerium für Bildung und Forschung (13GW0227B); European Research Council (646669); Deutsche Forschungsgemeinschaft (EXC 2167-390884018).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

Access to Document

10.1364/OL.424726

Cite this

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title = "Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm−1",

abstract = "Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500−1800 cm−1. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.",

author = "Hubertus Hakert and Matthias Eibl and Marie Tillich and Ralph Pries and Gereon H{\"u}ttmann and Ralf Brinkmann and Barbara Wollenberg and Bruchhage, \{Karl Ludwig\} and Sebastian Karpf and Robert Huber",

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T1 - Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm−1

AU - Hakert, Hubertus

AU - Eibl, Matthias

AU - Tillich, Marie

AU - Pries, Ralph

AU - Hüttmann, Gereon

AU - Brinkmann, Ralf

AU - Wollenberg, Barbara

AU - Bruchhage, Karl Ludwig

AU - Karpf, Sebastian

AU - Huber, Robert

PY - 2021/7/15

Y1 - 2021/7/15

N2 - Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500−1800 cm−1. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.

AB - Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500−1800 cm−1. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.

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