Thyroid hormones enhance hypoxia-induced erythropoietin production in vitro

J. Fandrey*, H. Pagel, S. Frede, M. Wolff, W. Jelkmann

*Corresponding author for this work
59 Citations (Scopus)


Effects of thyroid hormones on the production of erythropoietin (Epo) were investigated in isolated perfused rat kidneys and in the human hepatoma cell line, HepG2. Epo protein was measured by radioimmunoassay. L-triiodothyronine and L-thyroxine stimulated hypoxia-induced Epo formation both in the kidney and in hepG2 cells in a dose-dependent fashion. Quantitation of Epo mRNA by competitive polymerase chain reaction (PCR) showed that hypoxic HepG2 cells had three-fold higher Epo messenger RNA levels when treated with thyroid hormones for 3 hours. Measurements of oxygen consumption revealed that this effect was to due to an icnrease in the degree of hypoxia. Thus, apart from the known direct effect on erythroid precursors, thyroid hormones appear to stimulate erythropoiesis by a noncalorigenic increase in Epo production.

Original languageEnglish
JournalExperimental Hematology
Issue number3
Pages (from-to)272-277
Number of pages6
Publication statusPublished - 1994

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)


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