Three-dimensional orientational colocalization of individual donor-acceptor pairs

Christian G. Hübner; Vsevolod Ksenofontov; Fabian Nolde; Klaus Müllen; Thomas Basché

doi:10.1063/1.1760492

Three-dimensional orientational colocalization of individual donor-acceptor pairs

Christian G. Hübner^*, Vsevolod Ksenofontov, Fabian Nolde, Klaus Müllen, Thomas Basché

^*Corresponding author for this work

Institute of Physics

25 Citations (Scopus)

Abstract

The three-dimensional orientation of the donor and acceptor transition dipoles in fluorescence resonance energy transfer (FRET) pairs was discussed. The orientation of the dipoles was demonstrated by means of scanning optical microscopy with annular illumination. In this model system perylenediimide as the the donor and terryelenediimide as the acceptor were coupled via a stiff p-terphenyl linker. The results show that the application of annular illumination in conjuction with selective excitation of the donor and acceptor absorption transition dipoles in individual spFRET pairs allows for determination of their manual orientation.

Original language	English
Journal	Journal of Chemical Physics
Volume	120
Issue number	23
Pages (from-to)	10867-10870
Number of pages	4
ISSN	0021-9606
DOIs	https://doi.org/10.1063/1.1760492
Publication status	Published - 15.06.2004

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abstract = "The three-dimensional orientation of the donor and acceptor transition dipoles in fluorescence resonance energy transfer (FRET) pairs was discussed. The orientation of the dipoles was demonstrated by means of scanning optical microscopy with annular illumination. In this model system perylenediimide as the the donor and terryelenediimide as the acceptor were coupled via a stiff p-terphenyl linker. The results show that the application of annular illumination in conjuction with selective excitation of the donor and acceptor absorption transition dipoles in individual spFRET pairs allows for determination of their manual orientation.",

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AU - Hübner, Christian G.

AU - Ksenofontov, Vsevolod

AU - Nolde, Fabian

AU - Müllen, Klaus

AU - Basché, Thomas

PY - 2004/6/15

Y1 - 2004/6/15

N2 - The three-dimensional orientation of the donor and acceptor transition dipoles in fluorescence resonance energy transfer (FRET) pairs was discussed. The orientation of the dipoles was demonstrated by means of scanning optical microscopy with annular illumination. In this model system perylenediimide as the the donor and terryelenediimide as the acceptor were coupled via a stiff p-terphenyl linker. The results show that the application of annular illumination in conjuction with selective excitation of the donor and acceptor absorption transition dipoles in individual spFRET pairs allows for determination of their manual orientation.

AB - The three-dimensional orientation of the donor and acceptor transition dipoles in fluorescence resonance energy transfer (FRET) pairs was discussed. The orientation of the dipoles was demonstrated by means of scanning optical microscopy with annular illumination. In this model system perylenediimide as the the donor and terryelenediimide as the acceptor were coupled via a stiff p-terphenyl linker. The results show that the application of annular illumination in conjuction with selective excitation of the donor and acceptor absorption transition dipoles in individual spFRET pairs allows for determination of their manual orientation.

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DO - 10.1063/1.1760492

M3 - Journal articles

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VL - 120

SP - 10867

EP - 10870

JO - Journal of Chemical Physics

JF - Journal of Chemical Physics

IS - 23

ER -

Three-dimensional orientational colocalization of individual donor-acceptor pairs

Abstract

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