Abstract
The power of three-dimensional orientation detection of single emitting dipoles using a sophisticated scheme with three detectors in a confocal microscope is quantitatively explored by means of Monte Carlo simulations. We show that several hundreds of photons are sufficient for a reliable orientation determination. In typical single-molecule experiments, time resolutions in the submillisecond range for orientation trajectories become accessible. Experimental data on fluorescent latex beads and single perylene monoimide molecules show that a properly aligned setup can perfectly reproduce the simulated data. The simulations and experimental data highlight the potential of our method and give practical guidelines for its application.
Original language | English |
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Article number | 094703 |
Journal | Journal of Chemical Physics |
Volume | 129 |
Issue number | 9 |
ISSN | 0021-9606 |
DOIs | |
Publication status | Published - 15.09.2008 |