Abstract
Annealing kinetics of antisense species against two different target regions of the hepatitis B virus (HBV) were measured by kinetic in vitro selection. Individual association rates were related to energies calculated for local sequence segments and predicted structures of the complete pregenomic target RNA. A relationship between the presence of external loops and joint sequences with fast pairing was observed whereas internal loops did not favor fast RNA-RNA annealing. The findings were used to predict a fast-annealing HBV-directed antisense oligodeoxyribonucleotide that turned out to pair with its target RNA at an association rate constant of k = 9.2 x 104 M-1 S-1, which is substantially faster than the annealing rates of artificial antisense RNA so far included in in vitro selection assays.
Original language | English |
---|---|
Journal | Biological Chemistry |
Volume | 378 |
Issue number | 6 |
Pages (from-to) | 539-543 |
Number of pages | 5 |
ISSN | 1431-6730 |
DOIs | |
Publication status | Published - 06.1997 |