TY - JOUR
T1 - The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins
AU - Suvorova, Elena S.
AU - Duden, Rainer
AU - Lupashin, Vladimir V.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/5/13
Y1 - 2002/5/13
N2 - The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.
AB - The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.
UR - http://www.scopus.com/inward/record.url?scp=0037071543&partnerID=8YFLogxK
U2 - 10.1083/jcb.200111081
DO - 10.1083/jcb.200111081
M3 - Journal articles
C2 - 12011112
AN - SCOPUS:0037071543
SN - 0021-9525
VL - 157
SP - 631
EP - 643
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 4
ER -