The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins

Elena S. Suvorova, Rainer Duden, Vladimir V. Lupashin*

*Corresponding author for this work
169 Citations (Scopus)

Abstract

The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.

Original languageEnglish
JournalJournal of Cell Biology
Volume157
Issue number4
Pages (from-to)631-643
Number of pages13
ISSN0021-9525
DOIs
Publication statusPublished - 13.05.2002

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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