The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor

Frank J. Kaiser, Tarik Möröy, Glenn T.G. Chang, Bernhard Horsthemke, Hermann Josef Lüdecke*

*Corresponding author for this work
38 Citations (Scopus)

Abstract

The TRPS1 gene encodes a repressor of GATA-mediated transcription. Mutations in this gene cause the tricho-rhino-phalangeal syndromes, but the affected pathways are unknown. In a yeast two-hybrid screen with the C-terminal part of the murine Trps1 protein as bait, we obtained three yeast clones encoding two overlapping fragments of the 194 amino acids RING finger protein 4 (Rnf4). The overlap narrows down the Trps1-binding region within Rnf4 to amino acids 6-65. This region in Rnf4 is also known to interact with several proteins including steroid receptors. By using truncated Trps1 constructs, the Rnf4-binding region in Trps1 could be assigned to amino acids 985-1184 of 1281. This 200 amino acid region of Trps1 does not contain any predicted protein-protein interacting motif. Complex formation between the human proteins TRPS1 and RNF4 was verified by co-immunoprecipitation from transfected and native mammalian cells. Confocal laser-scanning microscopy revealed that the endogenous proteins are located in distinct structures of the nucleus. Using a luciferase reporter assay, we could demonstrate that the repressional function of TRPS1 is inhibited by RNF4. This finding suggests that RNF4 is a negative regulator of TRPS1 activity.

Original languageEnglish
JournalJournal of Biological Chemistry
Volume278
Issue number40
Pages (from-to)38780-38785
Number of pages6
ISSN0021-9258
DOIs
Publication statusPublished - 03.10.2003

Research Areas and Centers

  • Research Area: Medical Genetics

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