TY - JOUR
T1 - The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae
AU - Springer, Sebastian
AU - Chen, Eric
AU - Duden, Rainer
AU - Marzioch, Martina
AU - Rowley, Adele
AU - Hamamoto, Susan
AU - Merchant, Sabeeha
AU - Schekman, Randy
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/4/11
Y1 - 2000/4/11
N2 - To investigate the factors involved in the sorting of cargo proteins into COPII endoplasmic reticulum (ER) to Golgi apparatus transport vesicles, we have created a strain of S. cerevisiae (p24Δ8) that lacks all eight members of the p24 family of transmembrane proteins (Emp24p, Erv25p, and Erp1p to Erp6p). The p24 proteins have been implicated in COPI and COPII vesicle formation, cargo protein sorting, and regulation of vesicular transport in eukaryotic cells. We find that p24Δ8 cells grow identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to those seen in a single Δemp24 deletion strain. Thus, p24 proteins do not have an essential function in the secretory pathway. Instead, they may serve as quality control factors to restrict the entry of proteins into COPII vesicles.
AB - To investigate the factors involved in the sorting of cargo proteins into COPII endoplasmic reticulum (ER) to Golgi apparatus transport vesicles, we have created a strain of S. cerevisiae (p24Δ8) that lacks all eight members of the p24 family of transmembrane proteins (Emp24p, Erv25p, and Erp1p to Erp6p). The p24 proteins have been implicated in COPI and COPII vesicle formation, cargo protein sorting, and regulation of vesicular transport in eukaryotic cells. We find that p24Δ8 cells grow identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to those seen in a single Δemp24 deletion strain. Thus, p24 proteins do not have an essential function in the secretory pathway. Instead, they may serve as quality control factors to restrict the entry of proteins into COPII vesicles.
UR - http://www.scopus.com/inward/record.url?scp=0034635951&partnerID=8YFLogxK
U2 - 10.1073/pnas.070044097
DO - 10.1073/pnas.070044097
M3 - Journal articles
C2 - 10737764
AN - SCOPUS:0034635951
SN - 0027-8424
VL - 97
SP - 4034
EP - 4039
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -
