The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified

Reinhard Depping; Christiane Lohaus; Helmut E. Meyer; Wolfgang Rüger

doi:10.1016/j.bbrc.2005.08.023

The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified

Reinhard Depping^*, Christiane Lohaus, Helmut E. Meyer, Wolfgang Rüger

^*Corresponding author for this work

Institute of Physiology

Ruhr-University Bochum

43 Citations (Scopus)

Abstract

Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total ∼27) and ModB (total ∼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	335
Issue number	4
Pages (from-to)	1217-1223
Number of pages	7
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2005.08.023
Publication status	Published - 07.10.2005

Funding

The authors thank Jan Tkacz for a considerate discussion of the manuscript. We are grateful to E. Deuerling for her gift of purified E. coli trigger factor and to U. Aschke for excellent technical assistance. We gratefully acknowledge the financial support of the Deutsche Forschungsgemeinschaft by Grant RU 123-29-1.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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10.1016/j.bbrc.2005.08.023

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title = "The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified",

abstract = "Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total ∼27) and ModB (total ∼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.",

author = "Reinhard Depping and Christiane Lohaus and Meyer, \{Helmut E.\} and Wolfgang R{\"u}ger",

note = "Funding Information: The authors thank Jan Tkacz for a considerate discussion of the manuscript. We are grateful to E. Deuerling for her gift of purified E. coli trigger factor and to U. Aschke for excellent technical assistance. We gratefully acknowledge the financial support of the Deutsche Forschungsgemeinschaft by Grant RU 123-29-1. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Depping, Reinhard

AU - Lohaus, Christiane

AU - Meyer, Helmut E.

AU - Rüger, Wolfgang

N1 - Funding Information: The authors thank Jan Tkacz for a considerate discussion of the manuscript. We are grateful to E. Deuerling for her gift of purified E. coli trigger factor and to U. Aschke for excellent technical assistance. We gratefully acknowledge the financial support of the Deutsche Forschungsgemeinschaft by Grant RU 123-29-1. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/10/7

Y1 - 2005/10/7

N2 - Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total ∼27) and ModB (total ∼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

AB - Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total ∼27) and ModB (total ∼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

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SN - 0006-291X

VL - 335

SP - 1217

EP - 1223

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified

Abstract

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Research Areas and Centers

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