Abstract
The orientation of the membrane-attached cytochrome b558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 487 |
| Issue number | 3 |
| Pages (from-to) | 372-376 |
| Number of pages | 5 |
| ISSN | 0014-5793 |
| DOIs | |
| Publication status | Published - 05.01.2001 |
Funding
We thank the group of P. Bertrand for extensive access to the EPR facilities, as well as F. Lederer for stimulating discussions in the initial phases of this work. F. Baymann is supported by a FEBS long-term fellowship. M. Schütz benefitted from a post-doctoral position (‘chercheur associé’) by the CNRS.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
