The effects of ranibizumab (Lucentis) on retinal function in isolated perfused vertebrate retina

M. Lüke*, K. Januschowski, J. Lüke, S. Peters, N. Wirtz, E. Yörük, C. Lüke, K. U. Bartz-Schmidt, S. Grisanti, P. Szurman

*Corresponding author for this work
14 Citations (Scopus)


Background: Intraocular ranibizumab (Lucentis, Novartis, Basel Switzerland) is the primary choice in the treatment of neovascular age-related macular degeneration (AMD). Vascular endothelial growth factor (VEGF) is known to be a survival factor for neuronal cells. Therefore, blockage of all VEGF isoforms by ranibizumab could induce retinal dysfunction. Methods: Using isolated bovine retinas, the electroretinogram (ERG) was recorded as a transretinal potential using Ag/AgCl electrodes, while the retinas were perfused with an oxygen preincubated nutrient solution. For 45 min, ranibizumab was applied at a concentration of 0.2 mg/ml and alternatively the solvent carrier without the active agent. The ERG was monitored before, during and after exposure. Results: The concentration of 0.2 mg/ml ranibizumab induced a non-significant b-wave reduction of 22.32% after exposure (p=0.13). For the a-wave amplitude only a reduction of 4% was detected (p=0.18). The solvent carrier induced no significant reduction of the a- and bwave amplitudes (p=0.30 and p=0.979, respectively). Conclusion: In the ex vivo model, the isolated perfused vertebrate retina, ranibizumab has been proven to be a safe compound at the concentrations applied. The stability of the ERG-amplitudes rules out a considerable retinal dysfunction after an injection of up to 1 mg ranibizumab.

Original languageEnglish
JournalBritish Journal of Ophthalmology
Issue number10
Pages (from-to)1396-1400
Number of pages5
Publication statusPublished - 10.2009

Research Areas and Centers

  • Research Area: Luebeck Integrated Oncology Network (LION)


Dive into the research topics of 'The effects of ranibizumab (Lucentis) on retinal function in isolated perfused vertebrate retina'. Together they form a unique fingerprint.

Cite this