The effects of cetrorelix and triptorelin on the viability and steroidogenesis of cultured human granulosa luteinized cells

Chryssa Metallinou*, Frank Köster, Klaus Diedrich, Nikos Nikolettos, Byron Asimakopoulos

*Corresponding author for this work
5 Citations (Scopus)

Abstract

Background: We investigated the effects of the gonadotropin-releasing hormone (GnRH) agonist triptorelin as well the GnRH antagonist cetrorelix those of on the viability and steroidogenesis in human granulosa luteinized (hGL) cell cultures. Materials and Methods: The hGL cells were obtained from 34 women undergoing ovarian stimulation for IVF treatment. The cells were cultured for 48 h with or without 1 nM or 3 nM of cetrorelix or triptorelin in serum-free media. The cell viability was evaluated by the MTT [3-(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyl tetrazolium bromide] assay. The concentrations of estradiol and progesterone in culture supernatants were measured by ELISA. Results: Treatment with triptorelin slightly increased cell viability, whereas treatment with 3 nM cetrorelix led to a significant decrease. Estradiol concentrations were reduced with 3 nM triptorelin. Cultures treated with high-dose of either cetrorelix or triptorelin tended to secrete less progesterone than controls. Conclusion: Cetrorelix significantly reduces the viability of hGL cells. Triptorelin and cetrorelix may have minor effects on steroidogenesis. These results suggest that GnRH analogues may influence ovarian functions.

Original languageEnglish
JournalIn Vivo
Volume26
Issue number5
Pages (from-to)835-839
Number of pages5
ISSN0258-851X
Publication statusPublished - 01.09.2012

Research Areas and Centers

  • Research Area: Luebeck Integrated Oncology Network (LION)

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