The effect of anakinra on retinal function in isolated perfused vertebrate retina

Mahdy Ranjbar; Toni Schneider; Carl Brand; Salvatore Grisanti; Julia Lüke; Matthias Lüke

doi:10.1016/j.joco.2016.12.002

The effect of anakinra on retinal function in isolated perfused vertebrate retina

Mahdy Ranjbar, Toni Schneider, Carl Brand, Salvatore Grisanti, Julia Lüke, Matthias Lüke

Department of Ophtalmology

University of Cologne

4 Citations (Scopus)

Abstract

PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.

METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.

RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.

CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

Original language	English
Journal	Journal of current ophthalmology
Volume	29
Issue number	1
Pages (from-to)	69-71
Number of pages	3
ISSN	2452-2325
DOIs	https://doi.org/10.1016/j.joco.2016.12.002
Publication status	Published - 2017

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Cite this

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title = "The effect of anakinra on retinal function in isolated perfused vertebrate retina",

abstract = "PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret{\textregistered}), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.",

author = "Mahdy Ranjbar and Toni Schneider and Carl Brand and Salvatore Grisanti and Julia L{\"u}ke and Matthias L{\"u}ke",

year = "2017",

doi = "10.1016/j.joco.2016.12.002",

language = "English",

volume = "29",

pages = "69--71",

journal = "Journal of current ophthalmology",

issn = "2452-2325",

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T1 - The effect of anakinra on retinal function in isolated perfused vertebrate retina

AU - Ranjbar, Mahdy

AU - Schneider, Toni

AU - Brand, Carl

AU - Grisanti, Salvatore

AU - Lüke, Julia

AU - Lüke, Matthias

PY - 2017

Y1 - 2017

N2 - PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

AB - PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

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DO - 10.1016/j.joco.2016.12.002

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