The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

M. Wolff; J. Fandrey; J. Kalff; A. Muller; W. Jelkmann

The control of pericellular pO₂ in monolayer cell cultures: Microelectrode pO₂ measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

M. Wolff^*, J. Fandrey, J. Kalff, A. Muller, W. Jelkmann

^*Corresponding author for this work

Institute of Physiology

University of Bonn

Abstract

The control of pericellular pO₂ is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO₂ measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O₂ consumption and a diffusion- limited O₂ supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O₂ tensions.

Original language	English
Journal	Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft
Volume	6
Issue number	2
Pages (from-to)	159-161
Number of pages	3
ISSN	0935-1965
Publication status	Published - 1994

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Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Cite this

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title = "The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports",

abstract = "The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.",

author = "M. Wolff and J. Fandrey and J. Kalff and A. Muller and W. Jelkmann",

year = "1994",

language = "English",

volume = "6",

pages = "159--161",

journal = "Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft",

issn = "0935-1965",

publisher = "Pabst Science Publishers",

number = "2",

}

The control of pericellular pO₂ in monolayer cell cultures: Microelectrode pO₂ measurements in human hepatoma cell cultures on gas- permeable and impermeable supports. / Wolff, M.; Fandrey, J.; Kalff, J. et al.
In: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, Vol. 6, No. 2, 1994, p. 159-161.

Research output: Journal Articles › Journal articles › Research › peer-review

TY - JOUR

T1 - The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

AU - Wolff, M.

AU - Fandrey, J.

AU - Kalff, J.

AU - Muller, A.

AU - Jelkmann, W.

PY - 1994

Y1 - 1994

N2 - The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.

AB - The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.

UR - https://www.scopus.com/pages/publications/0028071499

M3 - Journal articles

AN - SCOPUS:0028071499

SN - 0935-1965

VL - 6

SP - 159

EP - 161

JO - Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft

JF - Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft

IS - 2

ER -

The control of pericellular pO₂ in monolayer cell cultures: Microelectrode pO₂ measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

Abstract

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