Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery

Nicole Pochert; Mariella Schneider; Melitta B Köpke; Mathis Wild; Angelika Mattmer; Jacqueline Sagasser; Monika M Golas; Maggie Banys-Paluchowski; Aline Metz; Christian Hinske; Matthias Reiger; Udo Jeschke; Christian Dannecker; Avidan Neumann; Claudia Traidl-Hoffmann; Michael Untch; Thorsten Kühn; Nina Ditsch

doi:10.1007/s00404-023-07074-w

Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery

Nicole Pochert, Mariella Schneider, Melitta B Köpke, Mathis Wild, Angelika Mattmer, Jacqueline Sagasser, Monika M Golas, Maggie Banys-Paluchowski, Aline Metz, Christian Hinske, Matthias Reiger, Udo Jeschke, Christian Dannecker, Avidan Neumann, Claudia Traidl-Hoffmann, Michael Untch, Thorsten Kühn, Nina Ditsch

Abstract

PURPOSE: The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6.

METHODS: Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls.

RESULTS: We found the Sf to be highly cytokine rich. Almost all analyzed cytokines were significantly higher in abundance in the Sf compared to Sp and Sc, especially IL-6, which promotes Th17 differentiation as well as suppresses Th1 differentiation in favor of Th2 development.

CONCLUSION: Our Sf cytokine measurements reflect a local immune event. In contrast, former study results on T-helper cell populations in both Sf and Sp tend to demonstrate a systemic immune process.

Original language	English
Journal	Archives of Gynecology and Obstetrics
Volume	308
Issue number	5
Pages (from-to)	1621-1627
Number of pages	7
ISSN	0932-0067
DOIs	https://doi.org/10.1007/s00404-023-07074-w
Publication status	Published - 2023

Research Areas and Centers

Centers: University Cancer Center Schleswig-Holstein (UCCSH)
Research Area: Luebeck Integrated Oncology Network (LION)
Academic Focus: Center for Infection and Inflammation Research (ZIEL)

DFG Research Classification Scheme

2.22-14 Hematology, Oncology
2.22-21 Gynaecology and Obstetrics
2.21-05 Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00404-023-07074-w

Cite this

Pochert, N., Schneider, M., Köpke, M. B., Wild, M., Mattmer, A., Sagasser, J., Golas, M. M., Banys-Paluchowski, M., Metz, A., Hinske, C., Reiger, M., Jeschke, U., Dannecker, C., Neumann, A., Traidl-Hoffmann, C., Untch, M., Kühn, T., & Ditsch, N. (2023). Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery. Archives of Gynecology and Obstetrics, 308(5), 1621-1627. https://doi.org/10.1007/s00404-023-07074-w

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title = "Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery",

abstract = "PURPOSE: The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6.METHODS: Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls.RESULTS: We found the Sf to be highly cytokine rich. Almost all analyzed cytokines were significantly higher in abundance in the Sf compared to Sp and Sc, especially IL-6, which promotes Th17 differentiation as well as suppresses Th1 differentiation in favor of Th2 development.CONCLUSION: Our Sf cytokine measurements reflect a local immune event. In contrast, former study results on T-helper cell populations in both Sf and Sp tend to demonstrate a systemic immune process.",

author = "Nicole Pochert and Mariella Schneider and K{\"o}pke, {Melitta B} and Mathis Wild and Angelika Mattmer and Jacqueline Sagasser and Golas, {Monika M} and Maggie Banys-Paluchowski and Aline Metz and Christian Hinske and Matthias Reiger and Udo Jeschke and Christian Dannecker and Avidan Neumann and Claudia Traidl-Hoffmann and Michael Untch and Thorsten K{\"u}hn and Nina Ditsch",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

doi = "10.1007/s00404-023-07074-w",

language = "English",

volume = "308",

pages = "1621--1627",

journal = "Archives of Gynecology and Obstetrics",

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Pochert, N, Schneider, M, Köpke, MB, Wild, M, Mattmer, A, Sagasser, J, Golas, MM, Banys-Paluchowski, M, Metz, A, Hinske, C, Reiger, M, Jeschke, U, Dannecker, C, Neumann, A, Traidl-Hoffmann, C, Untch, M, Kühn, T & Ditsch, N 2023, 'Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery', Archives of Gynecology and Obstetrics, vol. 308, no. 5, pp. 1621-1627. https://doi.org/10.1007/s00404-023-07074-w

TY - JOUR

T1 - Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery

AU - Pochert, Nicole

AU - Schneider, Mariella

AU - Köpke, Melitta B

AU - Wild, Mathis

AU - Mattmer, Angelika

AU - Sagasser, Jacqueline

AU - Golas, Monika M

AU - Banys-Paluchowski, Maggie

AU - Metz, Aline

AU - Hinske, Christian

AU - Reiger, Matthias

AU - Jeschke, Udo

AU - Dannecker, Christian

AU - Neumann, Avidan

AU - Traidl-Hoffmann, Claudia

AU - Untch, Michael

AU - Kühn, Thorsten

AU - Ditsch, Nina

PY - 2023

Y1 - 2023

N2 - PURPOSE: The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6.METHODS: Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls.RESULTS: We found the Sf to be highly cytokine rich. Almost all analyzed cytokines were significantly higher in abundance in the Sf compared to Sp and Sc, especially IL-6, which promotes Th17 differentiation as well as suppresses Th1 differentiation in favor of Th2 development.CONCLUSION: Our Sf cytokine measurements reflect a local immune event. In contrast, former study results on T-helper cell populations in both Sf and Sp tend to demonstrate a systemic immune process.

AB - PURPOSE: The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6.METHODS: Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls.RESULTS: We found the Sf to be highly cytokine rich. Almost all analyzed cytokines were significantly higher in abundance in the Sf compared to Sp and Sc, especially IL-6, which promotes Th17 differentiation as well as suppresses Th1 differentiation in favor of Th2 development.CONCLUSION: Our Sf cytokine measurements reflect a local immune event. In contrast, former study results on T-helper cell populations in both Sf and Sp tend to demonstrate a systemic immune process.

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DO - 10.1007/s00404-023-07074-w

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VL - 308

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EP - 1627

JO - Archives of Gynecology and Obstetrics

JF - Archives of Gynecology and Obstetrics

IS - 5

ER -

Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery

Abstract

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