TY - JOUR
T1 - Synthese kollagener und nicht-kollagener Proteine durch Zellen aus dem Parodont.
AU - Lang, H.
AU - Schüler, N.
AU - Zimmermann, A.
AU - Bodo, M.
AU - Brinckmann, J.
AU - Mertens, T.
AU - Müller, P. K.
AU - Nolden, R.
PY - 1992/12/1
Y1 - 1992/12/1
N2 - Synthesis of collagenous and noncollagenous proteins by cells originating from the alveolar bone (AB-cells) and from the periodontal ligament (PDL-cells) of 8 minipigs was analyzed in primary cell cultures. Incorporation of 3H-proline into proteins recovered from cell extracts showed, that 13.7% of protein synthesized by AB-cells and 8.0% of protein synthesized by PDL-cells were collagens. In both cell lines relative amounts of collagens synthesized decreased significantly on subculturing (AB-cells: 9.3%, PDL-cells: 6.0%). Preincubation of 2nd subculture AB-cells with beta-glycerophosphate had no significant effect on collagen synthesis. Considering the fact that relative amounts of collagen recovered from cell extracts represent only 10-20% of collagen recovered from whole culture (i.e. cells and culture medium), we suggest that collagen synthesis is an important component of in vitro-protein synthesis of cells originating from the alveolar bone and the periodontal ligament.
AB - Synthesis of collagenous and noncollagenous proteins by cells originating from the alveolar bone (AB-cells) and from the periodontal ligament (PDL-cells) of 8 minipigs was analyzed in primary cell cultures. Incorporation of 3H-proline into proteins recovered from cell extracts showed, that 13.7% of protein synthesized by AB-cells and 8.0% of protein synthesized by PDL-cells were collagens. In both cell lines relative amounts of collagens synthesized decreased significantly on subculturing (AB-cells: 9.3%, PDL-cells: 6.0%). Preincubation of 2nd subculture AB-cells with beta-glycerophosphate had no significant effect on collagen synthesis. Considering the fact that relative amounts of collagen recovered from cell extracts represent only 10-20% of collagen recovered from whole culture (i.e. cells and culture medium), we suggest that collagen synthesis is an important component of in vitro-protein synthesis of cells originating from the alveolar bone and the periodontal ligament.
UR - http://www.scopus.com/inward/record.url?scp=0027016153&partnerID=8YFLogxK
M3 - Zeitschriftenaufsätze
C2 - 1295586
AN - SCOPUS:0027016153
SN - 0940-855X
VL - 80
SP - 323
EP - 326
JO - Deutsche Zahn-, Mund-, und Kieferheilkunde mit Zentralblatt
JF - Deutsche Zahn-, Mund-, und Kieferheilkunde mit Zentralblatt
IS - 6
ER -
