TY - JOUR
T1 - Synergistic cooperation of Sall4 and Cyclin D1 in transcriptional repression
AU - Böhm, Johann
AU - Kaiser, Frank J.
AU - Borozdin, Wiktor
AU - Depping, Reinhard
AU - Kohlhase, Jürgen
N1 - Funding Information:
The authors thank Bernhard Horsthemke and Beate Albrecht for their support. This work was supported by the Deutsche Forschungsgemeinschaft (Grant DFG KO1850/6-2,3 to J.K.).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/5/11
Y1 - 2007/5/11
N2 - Loss of function mutations in SALL4 cause Okihiro syndrome, an autosomal dominant disorder characterised by radial ray malformations associated with Duane anomaly. In zebrafish and mouse Sall4 interacts with TBX5 during limb and heart development and plays a crucial role for embryonic stem (ES) cell pluripotency. Here we report the nuclear interaction of murine Sall4 with Cyclin D1, one of the main regulators of G1 to S phase transition in cell cycle, verified by yeast two-hybrid assay, co-immunoprecipitation and intracellular co-localisation. Furthermore, using luciferase reporter gene assays we demonstrate that Sall4 operates as a transcriptional repressor located to heterochromatin and that this activity is modulated by Cyclin D1.
AB - Loss of function mutations in SALL4 cause Okihiro syndrome, an autosomal dominant disorder characterised by radial ray malformations associated with Duane anomaly. In zebrafish and mouse Sall4 interacts with TBX5 during limb and heart development and plays a crucial role for embryonic stem (ES) cell pluripotency. Here we report the nuclear interaction of murine Sall4 with Cyclin D1, one of the main regulators of G1 to S phase transition in cell cycle, verified by yeast two-hybrid assay, co-immunoprecipitation and intracellular co-localisation. Furthermore, using luciferase reporter gene assays we demonstrate that Sall4 operates as a transcriptional repressor located to heterochromatin and that this activity is modulated by Cyclin D1.
UR - http://www.scopus.com/inward/record.url?scp=33947694647&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.03.050
DO - 10.1016/j.bbrc.2007.03.050
M3 - Journal articles
C2 - 17383611
AN - SCOPUS:33947694647
SN - 0006-291X
VL - 356
SP - 773
EP - 779
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
