TY - JOUR
T1 - Swimming training promotes cardiac remodeling and alters the expression of mRNA and protein levels involved in calcium handling in hypertensive rats
AU - Locatelli, Jamille
AU - De Assis, Leonardo Vinícius Monteiro
AU - Araújo, Carolina Morais
AU - Alzamora, Andréia Carvalho
AU - De Lima, Wanderson Geraldo
AU - Campagnole-Santos, Maria José
AU - Santos, Robson Augusto Dos
AU - Isoldi, Mauro César
N1 - Funding Information:
The research was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) , grants APQ-02112-10 and APQ 00793-13 ; RedeToxifar, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ) ; Instituto Nacional de Ciência e Tecnologia (INCT) ; and Programa de Apoio a Núcleos de Excelência . The co-author de Assis. L.V.M. is a Fellow of São Paulo Research Foundation (FAPESP-Process 2013/24337-4). All the authors are thankful to Pablo Henrique Oliveira e Silva for extensively revising this manuscript.
Publisher Copyright:
© 2014 Elsevier Inc. All rights reserved.
PY - 2014/11/11
Y1 - 2014/11/11
N2 - Aim The aim of this study was to identify the effects of swimming training on the mRNA expression and protein levels of the calcium handling proteins in the hearts of renovascular hypertensive rats submitted to swimming protocol during 6 weeks.Main methods Fischer rats with renovascular hypertension 2-kidney 1-clip (2K1C) and SHAM groups were divided among sedentary and exercised groups. The exercise protocol lasted for 6 weeks (1 h/day, 5×/week), and the mean arterial pressure, cardiomyocytes hypertrophy parameters, mRNA expression and protein levels of some calcium handling proteins in the left ventricle were evaluated.Key findings Swimming training was able to reduce the levels of mean arterial pressure in the hypertensive group compared to 2K1C SED, and to promote cardiac hypertrophy in SHAM EX and 2K1C EX groups in comparison to the respective control groups. The mRNA levels of B-type natriuretic peptide were reduced in the 2K1C EX when compared to 2K1C SED. The mRNA and protein levels of the sarcoplasmic reticulum Ca2 +-ATPase increased after the swimming training in SHAM and 2K1C groups. The mRNA and protein levels of phospholamban, displayed an increase in their levels in the exercised SHAM and in hypertensive rats in comparison to their respective controls; while mRNA levels of Na+/Ca2 + exchanger was reduced in the left ventricle comparing to the sedentary hypertensive rats.Significance Taken altogether, we provide evidence that the aerobic training may lead to cardiac remodeling, and modulate the calcium handling proteins expression in the heart of hypertensive rats.
AB - Aim The aim of this study was to identify the effects of swimming training on the mRNA expression and protein levels of the calcium handling proteins in the hearts of renovascular hypertensive rats submitted to swimming protocol during 6 weeks.Main methods Fischer rats with renovascular hypertension 2-kidney 1-clip (2K1C) and SHAM groups were divided among sedentary and exercised groups. The exercise protocol lasted for 6 weeks (1 h/day, 5×/week), and the mean arterial pressure, cardiomyocytes hypertrophy parameters, mRNA expression and protein levels of some calcium handling proteins in the left ventricle were evaluated.Key findings Swimming training was able to reduce the levels of mean arterial pressure in the hypertensive group compared to 2K1C SED, and to promote cardiac hypertrophy in SHAM EX and 2K1C EX groups in comparison to the respective control groups. The mRNA levels of B-type natriuretic peptide were reduced in the 2K1C EX when compared to 2K1C SED. The mRNA and protein levels of the sarcoplasmic reticulum Ca2 +-ATPase increased after the swimming training in SHAM and 2K1C groups. The mRNA and protein levels of phospholamban, displayed an increase in their levels in the exercised SHAM and in hypertensive rats in comparison to their respective controls; while mRNA levels of Na+/Ca2 + exchanger was reduced in the left ventricle comparing to the sedentary hypertensive rats.Significance Taken altogether, we provide evidence that the aerobic training may lead to cardiac remodeling, and modulate the calcium handling proteins expression in the heart of hypertensive rats.
UR - http://www.scopus.com/inward/record.url?scp=84908453209&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2014.09.024
DO - 10.1016/j.lfs.2014.09.024
M3 - Journal articles
C2 - 25283082
AN - SCOPUS:84908453209
SN - 0024-3205
VL - 117
SP - 67
EP - 74
JO - Life Sciences
JF - Life Sciences
IS - 2
ER -
