Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus

Christof Burgdorf; Laura Hänsel; Marc Heidbreder; Olaf Jöhren; Frank Schütte; Heribert Schunkert; Thomas Kurz

doi:10.1016/j.bbrc.2009.09.108

Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus

Christof Burgdorf^*, Laura Hänsel, Marc Heidbreder, Olaf Jöhren, Frank Schütte, Heribert Schunkert, Thomas Kurz

^*Corresponding author for this work

University of Kiel

26 Citations (Scopus)

Abstract

Lipin functions in mammalian phospholipid biosynthesis through its phosphatidate phosphohydrolase 1 (PAP₁) activity. Here, we studied cardiac PAP₁ activity and lipin expression ex vivo in 8-month-old Zucker diabetic fatty (ZDF) rats and humans with type 2 diabetes mellitus undergoing open heart surgery for coronary bypass grafting. Compared to non-diabetic littermates (ZDF-fa/+), left ventricular PAP₁ activity was 29% lower in diabetic ZDF-fa/fa rats. Left ventricular PAP₁ activities were 2.1-fold (ZDF-fa/fa) and 3.6-fold (ZDF-fa/+) higher than the respective atrial activities, indicating marked differences in cardiac distribution of PAP₁. PAP₁ activity was highly related with cardiac lipin-1 and lipin-3 mRNA expression in ZDF rats (r = 0.99 and 0.96). Consistent with the findings in experimental animals, human atrial tissue displayed PAP₁ activity that was 33% lower in those having diabetes than in non-diabetic controls. Accordingly, atrial lipin-1 and lipin-3 mRNA expression in diabetic patients was 50% and 59% lower as in non-diabetic patients, respectively. Insulin therapy increased both PAP₁ activity and lipin mRNA expression in diabetic patients. We conclude that suppression of cardiac PAP₁ activity/lipin expression may contribute to metabolic dysfunction of the diabetic heart.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	390
Issue number	1
Pages (from-to)	165-170
Number of pages	6
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2009.09.108
Publication status	Published - 04.12.2009

Funding

This study was supported by a grant of the Faculty of Medicine at the University of Lübeck (Forschungsförderung A40-2005 and E29-2009 ). We are very grateful to Ines Stölting, Anke Constanz, and Christine Eichholz for their technical assistance and to the team from the Department of Cardiac Surgery, University Hospital Schleswig-Holstein, Campus Lübeck, for cooperation in supplying atrial appendages from open heart surgery. Appendix A

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Access to Document

10.1016/j.bbrc.2009.09.108

Cite this

Burgdorf, C., Hänsel, L., Heidbreder, M., Jöhren, O., Schütte, F., Schunkert, H., & Kurz, T. (2009). Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus. Biochemical and Biophysical Research Communications, 390(1), 165-170. https://doi.org/10.1016/j.bbrc.2009.09.108

@article{b9a0363c436e4997add6a9256e6ef5e3,

title = "Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus",

abstract = "Lipin functions in mammalian phospholipid biosynthesis through its phosphatidate phosphohydrolase 1 (PAP1) activity. Here, we studied cardiac PAP1 activity and lipin expression ex vivo in 8-month-old Zucker diabetic fatty (ZDF) rats and humans with type 2 diabetes mellitus undergoing open heart surgery for coronary bypass grafting. Compared to non-diabetic littermates (ZDF-fa/+), left ventricular PAP1 activity was 29\% lower in diabetic ZDF-fa/fa rats. Left ventricular PAP1 activities were 2.1-fold (ZDF-fa/fa) and 3.6-fold (ZDF-fa/+) higher than the respective atrial activities, indicating marked differences in cardiac distribution of PAP1. PAP1 activity was highly related with cardiac lipin-1 and lipin-3 mRNA expression in ZDF rats (r = 0.99 and 0.96). Consistent with the findings in experimental animals, human atrial tissue displayed PAP1 activity that was 33\% lower in those having diabetes than in non-diabetic controls. Accordingly, atrial lipin-1 and lipin-3 mRNA expression in diabetic patients was 50\% and 59\% lower as in non-diabetic patients, respectively. Insulin therapy increased both PAP1 activity and lipin mRNA expression in diabetic patients. We conclude that suppression of cardiac PAP1 activity/lipin expression may contribute to metabolic dysfunction of the diabetic heart.",

author = "Christof Burgdorf and Laura H{\"a}nsel and Marc Heidbreder and Olaf J{\"o}hren and Frank Sch{\"u}tte and Heribert Schunkert and Thomas Kurz",

year = "2009",

month = dec,

day = "4",

doi = "10.1016/j.bbrc.2009.09.108",

language = "English",

volume = "390",

pages = "165--170",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

Burgdorf, C, Hänsel, L, Heidbreder, M, Jöhren, O, Schütte, F, Schunkert, H & Kurz, T 2009, 'Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus', Biochemical and Biophysical Research Communications, vol. 390, no. 1, pp. 165-170. https://doi.org/10.1016/j.bbrc.2009.09.108

Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus. / Burgdorf, Christof; Hänsel, Laura; Heidbreder, Marc et al.
In: Biochemical and Biophysical Research Communications, Vol. 390, No. 1, 04.12.2009, p. 165-170.

Research output: Journal Articles › Journal articles › Research › peer-review

TY - JOUR

T1 - Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus

AU - Burgdorf, Christof

AU - Hänsel, Laura

AU - Heidbreder, Marc

AU - Jöhren, Olaf

AU - Schütte, Frank

AU - Schunkert, Heribert

AU - Kurz, Thomas

PY - 2009/12/4

Y1 - 2009/12/4

N2 - Lipin functions in mammalian phospholipid biosynthesis through its phosphatidate phosphohydrolase 1 (PAP1) activity. Here, we studied cardiac PAP1 activity and lipin expression ex vivo in 8-month-old Zucker diabetic fatty (ZDF) rats and humans with type 2 diabetes mellitus undergoing open heart surgery for coronary bypass grafting. Compared to non-diabetic littermates (ZDF-fa/+), left ventricular PAP1 activity was 29% lower in diabetic ZDF-fa/fa rats. Left ventricular PAP1 activities were 2.1-fold (ZDF-fa/fa) and 3.6-fold (ZDF-fa/+) higher than the respective atrial activities, indicating marked differences in cardiac distribution of PAP1. PAP1 activity was highly related with cardiac lipin-1 and lipin-3 mRNA expression in ZDF rats (r = 0.99 and 0.96). Consistent with the findings in experimental animals, human atrial tissue displayed PAP1 activity that was 33% lower in those having diabetes than in non-diabetic controls. Accordingly, atrial lipin-1 and lipin-3 mRNA expression in diabetic patients was 50% and 59% lower as in non-diabetic patients, respectively. Insulin therapy increased both PAP1 activity and lipin mRNA expression in diabetic patients. We conclude that suppression of cardiac PAP1 activity/lipin expression may contribute to metabolic dysfunction of the diabetic heart.

AB - Lipin functions in mammalian phospholipid biosynthesis through its phosphatidate phosphohydrolase 1 (PAP1) activity. Here, we studied cardiac PAP1 activity and lipin expression ex vivo in 8-month-old Zucker diabetic fatty (ZDF) rats and humans with type 2 diabetes mellitus undergoing open heart surgery for coronary bypass grafting. Compared to non-diabetic littermates (ZDF-fa/+), left ventricular PAP1 activity was 29% lower in diabetic ZDF-fa/fa rats. Left ventricular PAP1 activities were 2.1-fold (ZDF-fa/fa) and 3.6-fold (ZDF-fa/+) higher than the respective atrial activities, indicating marked differences in cardiac distribution of PAP1. PAP1 activity was highly related with cardiac lipin-1 and lipin-3 mRNA expression in ZDF rats (r = 0.99 and 0.96). Consistent with the findings in experimental animals, human atrial tissue displayed PAP1 activity that was 33% lower in those having diabetes than in non-diabetic controls. Accordingly, atrial lipin-1 and lipin-3 mRNA expression in diabetic patients was 50% and 59% lower as in non-diabetic patients, respectively. Insulin therapy increased both PAP1 activity and lipin mRNA expression in diabetic patients. We conclude that suppression of cardiac PAP1 activity/lipin expression may contribute to metabolic dysfunction of the diabetic heart.

UR - https://www.scopus.com/pages/publications/70349947074

U2 - 10.1016/j.bbrc.2009.09.108

DO - 10.1016/j.bbrc.2009.09.108

M3 - Journal articles

C2 - 19799857

AN - SCOPUS:70349947074

SN - 0006-291X

VL - 390

SP - 165

EP - 170

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Suppression of cardiac phosphatidate phosphohydrolase 1 activity and lipin mRNA expression in Zucker diabetic fatty rats and humans with type 2 diabetes mellitus

Abstract

Funding

UN SDGs

Research Areas and Centers

Access to Document

Other files and links

Fingerprint

Cite this