31P-NMR spectra of the Ha-ras p21. Nucleotide complexes

P. Rösch*, A. Wittinghofer, J. Tucker, G. Sczakiel, R. Leberman, I. Schlichting

*Corresponding author for this work
14 Citations (Scopus)


Phosphorus nuclear magnetic resonance spectra of the Ha-ras oncogene product p21 and its nucleotide complexes have been obtained. It is shown that the 31P nuclear magnetic resonance spectra of a number of nucleotide-enzyme complexes show some common features. In particular, the chemical shift values of the β-phosphorus resonance of enzyme-bound NTP and NDP (N=A,G) of hydrolases exhibit a downfield shift virtually identical for myosin, elongation factor Tu, and the Ha-ras oncogene product p21. This suggests that the stereochemistry around the β-phosphorus might be similar in these compounds.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Issue number2
Pages (from-to)549-555
Number of pages7
Publication statusPublished - 13.03.1986


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