TY - JOUR
T1 - Subepidermal blistering disease with autoantibodies against a novel dermal 200-kDa antigen
AU - Kawahara, Yoshie
AU - Zillikens, Detlef
AU - Yancey, Kim B.
AU - Marinkovich, M. Peter
AU - Nie, Zhuxiang
AU - Hashimoto, Takashi
AU - Nishikawa, Takeji
N1 - Funding Information:
We thank Dr W. Salmhofer and Professor H.P. Soyer, University of Graz, B.S. Bhogal and Dr M.M. Black, St Thomas’ Hospital, Dr S. Shirahama, Hamamatsu University School of Medicine, and Dr K.R Chen, Ogikubo Hospital, Tokyo for providing us with sera used in this study. We are also grateful for the assistance from the Office of Research and Development, VA Palo Alto Health Care System. This work was supported in part by Grant-in-Aid of Scientific Research from the Ministry of Education, Science and Culture of Japan, and Grant-in-NIH AR 44012.
PY - 2000/6
Y1 - 2000/6
N2 - A number of autoimmune subepidermal blistering diseases are characterized by the distinct autoantigens of the cutaneous basement membrane zone. Recently, a few cases with autoantibodies against a novel 200-kDa dermal protein have been reported. We collected nine cases of subepidermal blistering disease with IgG antibodies against this 200-kDa antigen. In this report, we describe the clinical and immunological appearances in these cases. Five cases showed bullous pemphigoid-like features, one case resembled dermatitis herpetiformis, and another case showed mixed features of bullous pemphigoid and linear IgA bullous dermatosis. It was interesting to note that psoriasis coexisted in four cases. By indirect immunofluorescence on 1 M NaCl split skin, IgG antibodies from all sera reacted with the dermal side of the split. By immunoblot analysis, IgG antibodies recognized a 200-kDa protein of dermal extract. IgG affinity-purified antibodies on the 200-kDa immunoblot membrane stained the dermal side of 1 M NaCl split skin. Various examinations suggested that the 200-kDa antigen is not identical to any chains of laminins-1, -5 or -6. This autoimmune subepidermal blistering disease against the dermal 200-kDa protein may form a new distinct entity, which often associates with psoriasis. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - A number of autoimmune subepidermal blistering diseases are characterized by the distinct autoantigens of the cutaneous basement membrane zone. Recently, a few cases with autoantibodies against a novel 200-kDa dermal protein have been reported. We collected nine cases of subepidermal blistering disease with IgG antibodies against this 200-kDa antigen. In this report, we describe the clinical and immunological appearances in these cases. Five cases showed bullous pemphigoid-like features, one case resembled dermatitis herpetiformis, and another case showed mixed features of bullous pemphigoid and linear IgA bullous dermatosis. It was interesting to note that psoriasis coexisted in four cases. By indirect immunofluorescence on 1 M NaCl split skin, IgG antibodies from all sera reacted with the dermal side of the split. By immunoblot analysis, IgG antibodies recognized a 200-kDa protein of dermal extract. IgG affinity-purified antibodies on the 200-kDa immunoblot membrane stained the dermal side of 1 M NaCl split skin. Various examinations suggested that the 200-kDa antigen is not identical to any chains of laminins-1, -5 or -6. This autoimmune subepidermal blistering disease against the dermal 200-kDa protein may form a new distinct entity, which often associates with psoriasis. Copyright (C) 2000 Elsevier Science Ireland Ltd.
UR - http://www.scopus.com/inward/record.url?scp=0034213798&partnerID=8YFLogxK
U2 - 10.1016/S0923-1811(99)00093-6
DO - 10.1016/S0923-1811(99)00093-6
M3 - Journal articles
C2 - 10808126
AN - SCOPUS:0034213798
SN - 0923-1811
VL - 23
SP - 93
EP - 102
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
IS - 2
ER -