Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions

Daniel Weng; Hubertus Hakert; Torben Blömker; Jan Philip Kolb; Matthias Strauch; Matthias Eibl; Philipp Lamminger; Sebastian Karpf; Robert Huber

doi:10.1109/CLEOE-EQEC.2019.8872571

Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions

Daniel Weng, Hubertus Hakert, Torben Blömker, Jan Philip Kolb, Matthias Strauch, Matthias Eibl, Philipp Lamminger, Sebastian Karpf, Robert Huber

Abstract

Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan [1].

Original language	English
Title of host publication	2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)
Publisher	IEEE
Publication date	06.2019
Article number	8872571
ISBN (Print)	978-1-7281-0470-6
ISBN (Electronic)	978-1-7281-0469-0
DOIs	https://doi.org/10.1109/CLEOE-EQEC.2019.8872571
Publication status	Published - 06.2019
Event	2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference - Munich, Germany Duration: 23.06.2019 → 27.06.2019 Conference number: 152846

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Academic Focus: Biomedical Engineering

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10.1109/CLEOE-EQEC.2019.8872571

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Weng, D., Hakert, H., Blömker, T., Kolb, J. P., Strauch, M., Eibl, M., Lamminger, P., Karpf, S., & Huber, R. (2019). Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions. In 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) Article 8872571 IEEE. https://doi.org/10.1109/CLEOE-EQEC.2019.8872571

@inproceedings{bb5804fef92f4d12a1cf3ac5a9c840b3,

title = "Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions",

abstract = "Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan [1].",

author = "Daniel Weng and Hubertus Hakert and Torben Bl{\"o}mker and Kolb, {Jan Philip} and Matthias Strauch and Matthias Eibl and Philipp Lamminger and Sebastian Karpf and Robert Huber",

note = "Funding Information: The project was funded by the DFG (HU1006/6&EXC2167), the European Union (ERC CoG 646669), Interreg DE-DK regional fund “CELLTOM”, the BMBF (13GW0227B&13N14665). Publisher Copyright: {\textcopyright} 2019 IEEE. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.; 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019 ; Conference date: 23-06-2019 Through 27-06-2019",

year = "2019",

month = jun,

doi = "10.1109/CLEOE-EQEC.2019.8872571",

language = "English",

isbn = "978-1-7281-0470-6",

booktitle = "2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)",

publisher = "IEEE",

}

Weng, D, Hakert, H, Blömker, T, Kolb, JP , Strauch, M, Eibl, M, Lamminger, P, Karpf, S & Huber, R 2019, Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions. in 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)., 8872571, IEEE, 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, Munich, Bavaria, Germany, 23.06.19. https://doi.org/10.1109/CLEOE-EQEC.2019.8872571

Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions. / Weng, Daniel; Hakert, Hubertus; Blömker, Torben et al.
2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC). IEEE, 2019. 8872571.

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

TY - GEN

T1 - Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions

AU - Weng, Daniel

AU - Hakert, Hubertus

AU - Blömker, Torben

AU - Kolb, Jan Philip

AU - Strauch, Matthias

AU - Eibl, Matthias

AU - Lamminger, Philipp

AU - Karpf, Sebastian

AU - Huber, Robert

N1 - Conference code: 152846

PY - 2019/6

Y1 - 2019/6

N2 - Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan [1].

AB - Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan [1].

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U2 - 10.1109/CLEOE-EQEC.2019.8872571

DO - 10.1109/CLEOE-EQEC.2019.8872571

M3 - Conference contribution

SN - 978-1-7281-0470-6

BT - 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)

PB - IEEE

T2 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference

Y2 - 23 June 2019 through 27 June 2019

ER -

Sub-nanosecond pulsed fiber laser for 532nm two-photon excitation fluorescence (TPEF) microscopy of UV transitions

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