Structure of the X (ADRP) domain of nsp3 from feline coronavirus

Justyna A. Wojdyla; Ioannis Manolaridis; Eric J. Snijder; Alexander E. Gorbalenya; Bruno Coutard; Yvonne Piotrowski; Rolf Hilgenfeld; Paul A. Tucker

doi:10.1107/S0907444909040074

Structure of the X (ADRP) domain of nsp3 from feline coronavirus

Justyna A. Wojdyla, Ioannis Manolaridis, Eric J. Snijder, Alexander E. Gorbalenya, Bruno Coutard, Yvonne Piotrowski, Rolf Hilgenfeld, Paul A. Tucker

Institute of Biochemistry

16 Citations (Scopus)

Abstract

The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

Original language	English
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	65
Issue number	12
Pages (from-to)	1292-1300
Number of pages	9
ISSN	0907-4449
DOIs	https://doi.org/10.1107/S0907444909040074
Publication status	Published - 18.12.2009

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1107/S0907444909040074

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title = "Structure of the X (ADRP) domain of nsp3 from feline coronavirus",

abstract = "The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 {\AA} resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70\%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 {\AA} resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.",

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T1 - Structure of the X (ADRP) domain of nsp3 from feline coronavirus

AU - Wojdyla, Justyna A.

AU - Manolaridis, Ioannis

AU - Snijder, Eric J.

AU - Gorbalenya, Alexander E.

AU - Coutard, Bruno

AU - Piotrowski, Yvonne

AU - Hilgenfeld, Rolf

AU - Tucker, Paul A.

PY - 2009/12/18

Y1 - 2009/12/18

N2 - The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

AB - The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

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JF - Acta Crystallographica Section D: Biological Crystallography

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Structure of the X (ADRP) domain of nsp3 from feline coronavirus

Abstract

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