TY - JOUR
T1 - Structure of the X (ADRP) domain of nsp3 from feline coronavirus
AU - Wojdyla, Justyna A.
AU - Manolaridis, Ioannis
AU - Snijder, Eric J.
AU - Gorbalenya, Alexander E.
AU - Coutard, Bruno
AU - Piotrowski, Yvonne
AU - Hilgenfeld, Rolf
AU - Tucker, Paul A.
PY - 2009/12/18
Y1 - 2009/12/18
N2 - The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
AB - The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol-3-phos-phate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
UR - http://www.scopus.com/inward/record.url?scp=71949089310&partnerID=8YFLogxK
U2 - 10.1107/S0907444909040074
DO - 10.1107/S0907444909040074
M3 - Journal articles
C2 - 19966415
AN - SCOPUS:71949089310
SN - 0907-4449
VL - 65
SP - 1292
EP - 1300
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -