TY - JOUR
T1 - Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue
AU - Szymanski, Kolja V.
AU - Toennies, Mario
AU - Becher, Anne
AU - Fatykhova, Diana
AU - N'Guessan, Philippe D.
AU - Gutbier, Birgitt
AU - Klauschen, Frederick
AU - Neuschaefer-Rube, Frank
AU - Schneider, Paul
AU - Rueckert, Jens
AU - Neudecker, Jens
AU - Bauer, Torsten T.
AU - Dalhoff, Klaus
AU - Drom̈ann, Daniel
AU - Gruber, Achim D.
AU - Kershaw, Olivia
AU - Temmesfeld-Wollbrueck, Bettina
AU - Suttorp, Norbert
AU - Hippenstiel, Stefan
AU - Hocke, Andreas C.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid4receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections. Copyright
AB - The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid4receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections. Copyright
UR - http://www.scopus.com/inward/record.url?scp=84871238582&partnerID=8YFLogxK
U2 - 10.1183/09031936.00186911
DO - 10.1183/09031936.00186911
M3 - Journal articles
C2 - 22441740
AN - SCOPUS:84871238582
SN - 0903-1936
VL - 40
SP - 1458
EP - 1467
JO - European Respiratory Journal
JF - European Respiratory Journal
IS - 6
ER -
