Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas

Matthias H.F. Klinger; Andrés S. Mendoza; Harald Klüter; Heinz Jürgen Krammer; Wolfgang Kühnel

doi:10.1007/BF00343945

Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas

Matthias H.F. Klinger^*, Andrés S. Mendoza, Harald Klüter, Heinz Jürgen Krammer, Wolfgang Kühnel

^*Corresponding author for this work

Institute of Anatomy

9 Citations (Scopus)

Abstract

We report the ultrastructural changes occurring in human platelets during eight days of storage. Extension of pseudopodia is frequently observed, but a concentration of organelles in the centre of the platelets is found only in a minor fraction (∼5%). Striking changes can be observed in both the granules and the open canalicular system. In fresh platelets, the latter often has the form of stacked membranes that have no lumen, but these membranes separate and spread with increasing storage time. However, the openings of this system on the outer surface of the platelet remain unchanged. Some of these features differ from the morphological description of platelets activated by thrombin or ADP, and suggest that the storage lesion is the result of a prolonged weak activation that leads to an incomplete release reaction within the first five days.

Original language	English
Journal	Cell & Tissue Research
Volume	276
Issue number	3
Pages (from-to)	477-483
Number of pages	7
ISSN	0302-766X
DOIs	https://doi.org/10.1007/BF00343945
Publication status	Published - 06.1994

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Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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title = "Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas",

abstract = "We report the ultrastructural changes occurring in human platelets during eight days of storage. Extension of pseudopodia is frequently observed, but a concentration of organelles in the centre of the platelets is found only in a minor fraction (∼5\%). Striking changes can be observed in both the granules and the open canalicular system. In fresh platelets, the latter often has the form of stacked membranes that have no lumen, but these membranes separate and spread with increasing storage time. However, the openings of this system on the outer surface of the platelet remain unchanged. Some of these features differ from the morphological description of platelets activated by thrombin or ADP, and suggest that the storage lesion is the result of a prolonged weak activation that leads to an incomplete release reaction within the first five days.",

author = "Klinger, \{Matthias H.F.\} and Mendoza, \{Andr{\'e}s S.\} and Harald Kl{\"u}ter and Krammer, \{Heinz J{\"u}rgen\} and Wolfgang K{\"u}hnel",

year = "1994",

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T1 - Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas

AU - Klinger, Matthias H.F.

AU - Mendoza, Andrés S.

AU - Klüter, Harald

AU - Krammer, Heinz Jürgen

AU - Kühnel, Wolfgang

PY - 1994/6

Y1 - 1994/6

N2 - We report the ultrastructural changes occurring in human platelets during eight days of storage. Extension of pseudopodia is frequently observed, but a concentration of organelles in the centre of the platelets is found only in a minor fraction (∼5%). Striking changes can be observed in both the granules and the open canalicular system. In fresh platelets, the latter often has the form of stacked membranes that have no lumen, but these membranes separate and spread with increasing storage time. However, the openings of this system on the outer surface of the platelet remain unchanged. Some of these features differ from the morphological description of platelets activated by thrombin or ADP, and suggest that the storage lesion is the result of a prolonged weak activation that leads to an incomplete release reaction within the first five days.

AB - We report the ultrastructural changes occurring in human platelets during eight days of storage. Extension of pseudopodia is frequently observed, but a concentration of organelles in the centre of the platelets is found only in a minor fraction (∼5%). Striking changes can be observed in both the granules and the open canalicular system. In fresh platelets, the latter often has the form of stacked membranes that have no lumen, but these membranes separate and spread with increasing storage time. However, the openings of this system on the outer surface of the platelet remain unchanged. Some of these features differ from the morphological description of platelets activated by thrombin or ADP, and suggest that the storage lesion is the result of a prolonged weak activation that leads to an incomplete release reaction within the first five days.

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DO - 10.1007/BF00343945

M3 - Journal articles

C2 - 8062341

AN - SCOPUS:0028239514

SN - 0302-766X

VL - 276

SP - 477

EP - 483

JO - Cell & Tissue Research

JF - Cell & Tissue Research

IS - 3

ER -

Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas

Abstract

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