Stellenwert der Vitrifikation in der Reproduktionsmedizin

Vitrification is a relatively new technique for the cryopreservation of cells and tissues. A key element of vitrification is the direct transformation of cells from a fluid state into an amorphous, vitrified state without the formation of ice crystals. Vitrification may be especially useful in the field of reproductive medicine. Compared to the more established method of slow freezing, vitrification seems to be more suitable for cryobiologically difficult to freeze cells, tissues and developmental stages. Unfertilized metaphase II oocytes and blastocysts show very high survival and developmental rates after vitrification. Recent improvements allow for aseptic vitrification by avoiding direct contact between the specimen and liquid nitrogen. As with every new laboratory technique, the introduction of vitrification into the the laboratory requires the careful application of all technical details in order to achieve its full potential.