TY - JOUR
T1 - Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish
AU - Ohnmacht, Jochen
AU - Yang, Yujie
AU - Maurer, Gianna W.
AU - Barreiro-Iglesias, Antón
AU - Tsarouchas, Themistoklis M.
AU - Wehner, Daniel
AU - Sieger, Dirk
AU - Becker, Catherina G.
AU - Becker, Thomas
PY - 2016/5/1
Y1 - 2016/5/1
N2 - In adult zebrafish, relatively quiescent progenitor cells show lesioninduced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 h of a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. By contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Either mechanical lesions or motor neuron ablation is sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
AB - In adult zebrafish, relatively quiescent progenitor cells show lesioninduced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 h of a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. By contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Either mechanical lesions or motor neuron ablation is sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
UR - http://www.scopus.com/inward/record.url?scp=84964821690&partnerID=8YFLogxK
U2 - 10.1242/dev.129155
DO - 10.1242/dev.129155
M3 - Journal articles
C2 - 26965370
AN - SCOPUS:84964821690
SN - 0950-1991
VL - 143
SP - 1464
EP - 1474
JO - Development (Cambridge)
JF - Development (Cambridge)
IS - 9
ER -