Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region

Georg Sczakiel; MICHAEL PAWLITA; Andreas Kleinheinz

doi:10.1016/0006-291X(90)90379-2

Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region

Georg Sczakiel^*, MICHAEL PAWLITA, Andreas Kleinheinz

^*Corresponding author for this work

German Cancer Research Center

38 Citations (Scopus)

Abstract

The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4⁺ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	2
Pages (from-to)	643-651
Number of pages	9
ISSN	0006-291X
DOIs	https://doi.org/10.1016/0006-291X(90)90379-2
Publication status	Published - 15.06.1990

Funding

We thank H. xur Hausen and L. Giimann for stimulating discussions and continuous encouragement. We also thank M. Martin for providing HIV-l proviral clone pNL4-3, R.C. GaIIo for HIV-l clone pBH10, H.-P. Vosberg for plasmid pHIV410 and H. Stijppler for plasmid pCMV-CAT. We thank A. Henn and H. G6txmann for excellent technical assistance and V. Bosch and H.-G. Rrgusslich for criticaIIy reading this manuscript. This work was supported by BMFf grant FKZ B-083-89.

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SDG 3 Good Health and Well-being

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10.1016/0006-291X(90)90379-2

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title = "Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region",

abstract = "The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4+ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70\%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.",

author = "Georg Sczakiel and MICHAEL PAWLITA and Andreas Kleinheinz",

note = "Funding Information: We thank H. xur Hausen and L. Giimann for stimulating discussions and continuous encouragement. We also thank M. Martin for providing HIV-l proviral clone pNL4-3, R.C. GaIIo for HIV-l clone pBH10, H.-P. Vosberg for plasmid pHIV410 and H. Stijppler for plasmid pCMV-CAT. We thank A. Henn and H. G6txmann for excellent technical assistance and V. Bosch and H.-G. Rrgusslich for criticaIIy reading this manuscript. This work was supported by BMFf grant FKZ B-083-89.",

year = "1990",

month = jun,

day = "15",

doi = "10.1016/0006-291X(90)90379-2",

language = "English",

volume = "169",

pages = "643--651",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

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Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region. / Sczakiel, Georg; PAWLITA, MICHAEL; Kleinheinz, Andreas.
In: Biochemical and Biophysical Research Communications, Vol. 169, No. 2, 15.06.1990, p. 643-651.

Research output: Journal Articles › Journal articles › Research › peer-review

TY - JOUR

T1 - Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region

AU - Sczakiel, Georg

AU - PAWLITA, MICHAEL

AU - Kleinheinz, Andreas

N1 - Funding Information: We thank H. xur Hausen and L. Giimann for stimulating discussions and continuous encouragement. We also thank M. Martin for providing HIV-l proviral clone pNL4-3, R.C. GaIIo for HIV-l clone pBH10, H.-P. Vosberg for plasmid pHIV410 and H. Stijppler for plasmid pCMV-CAT. We thank A. Henn and H. G6txmann for excellent technical assistance and V. Bosch and H.-G. Rrgusslich for criticaIIy reading this manuscript. This work was supported by BMFf grant FKZ B-083-89.

PY - 1990/6/15

Y1 - 1990/6/15

N2 - The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4+ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.

AB - The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4+ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.

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JF - Biochemical and Biophysical Research Communications

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ER -

Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region

Abstract

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