Abstract
Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of a sample. However, a more widespread application of this technique is hindered by the need for a sophisticated ultra-short pulse laser source and by speed limitations of current FLIM detection systems. To overcome these limitations, we combined a robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth detection. Due to the long pulse length in our configuration, more fluorescence photons are generated per pulse, which allows us to derive the lifetime with a single excitation pulse only. In this paper, we show high quality FLIM images acquired at a pixel rate of 1 MHz. This approach is a promising candidate for an easy-to-use and benchtop FLIM system to make this technique available to a wider research community.
| Original language | English |
|---|---|
| Article number | 288056 |
| Journal | Biomedical Optics Express |
| Volume | 8 |
| Issue number | 7 |
| Pages (from-to) | 3132-3142 |
| Number of pages | 11 |
| DOIs | |
| Publication status | Published - 01.07.2017 |
Funding
European Union project ENCOMOLE-2i (Horizon 2020, ERC CoG no. 646669); German Research Foundation (DFG project HU1006/6 and EXC 306/2). We would like to acknowledge support from A. Vogel at the Institute of Biomedical Optics (BMO) at the University of L?beck. Moreover we would like to thank Gereon H?ttmann (BMO) for helpful discussions on multi-photon imaging technology and Astrid Link and Ramtin Rahmanzadeh (both BMO), Christin Lehmann (Institute for anatomy), and Zouhair Aherrahrou (Institute for Integrative and Experimental Genomics) for fruitful discussions on cell imaging and help with sample preparation.
Research Areas and Centers
- Academic Focus: Biomedical Engineering
