Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid

Jan Rupp; Werner Solbach; Jens Gieffers

doi:10.1128/AEM.72.5.3785-3787.2006

Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid

Jan Rupp, Werner Solbach, Jens Gieffers^*

^*Corresponding author for this work

17 Citations (Scopus)

Abstract

Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	5
Pages (from-to)	3785-3787
Number of pages	3
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.72.5.3785-3787.2006
Publication status	Published - 01.05.2006

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1128/AEM.72.5.3785-3787.2006

Cite this

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AU - Solbach, Werner

AU - Gieffers, Jens

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N2 - Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

AB - Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

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U2 - 10.1128/AEM.72.5.3785-3787.2006

DO - 10.1128/AEM.72.5.3785-3787.2006

M3 - Journal articles

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Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid

Abstract

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