TY - JOUR
T1 - Simultaneous eicosanoid profiling and identification by liquid chromatography and hybrid triple quadrupole-linear ion trap mass spectrometry for metabolomic studies in human plasma
AU - Kortz, Linda
AU - Geyer, Roland
AU - Ludwig, Ute
AU - Planert, Mathis
AU - Bruegel, Mathias
AU - Leichtle, Alexander
AU - Fiedler, Georg Martin
AU - Thiery, Joachim
AU - Ceglarek, Uta
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2009/11
Y1 - 2009/11
N2 - Eicosanoids play a key role in many physiological and pathological processes and might therefore serve as interesting diagnostic targets. Methods for the analysis of arachidonic acid metabolites in cells and body fluids require high sensitivity and specificity because of the very low concentrations, similar chemical structures and short half-lives of these metabolites. We established a mass spectrometric method for the simultaneous identification and quantification of arachidonic acid metabolites in human plasma samples using solid phase extraction followed by liquid chromatography (LC) and hybrid triple quadrupole-linear ion trap (QqLIT) mass spectrometry. Quantitative analysis was performed using the 4000 QTrap tandem mass spectrometer in multiple reaction monitoring (MRM) mode. As part of an independent data acquisition experiment MRMs were used as survey scans, which dependently triggered enhanced product ion (EPI) scans. Compound identification was carried out by library search using a library based on EPI spectra of standard components (prostaglandins, thromboxanes, leukotrienes and isoprostanes). The newly developed compound library enables the verification of known and structural elucidation of unknown eicosanoid metabolites in human plasma. In conclusion, our mass spectrometric method allows the simultaneous identification and quantification of arachidonic acid metabolites in one single LC-MS/MS run.
AB - Eicosanoids play a key role in many physiological and pathological processes and might therefore serve as interesting diagnostic targets. Methods for the analysis of arachidonic acid metabolites in cells and body fluids require high sensitivity and specificity because of the very low concentrations, similar chemical structures and short half-lives of these metabolites. We established a mass spectrometric method for the simultaneous identification and quantification of arachidonic acid metabolites in human plasma samples using solid phase extraction followed by liquid chromatography (LC) and hybrid triple quadrupole-linear ion trap (QqLIT) mass spectrometry. Quantitative analysis was performed using the 4000 QTrap tandem mass spectrometer in multiple reaction monitoring (MRM) mode. As part of an independent data acquisition experiment MRMs were used as survey scans, which dependently triggered enhanced product ion (EPI) scans. Compound identification was carried out by library search using a library based on EPI spectra of standard components (prostaglandins, thromboxanes, leukotrienes and isoprostanes). The newly developed compound library enables the verification of known and structural elucidation of unknown eicosanoid metabolites in human plasma. In conclusion, our mass spectrometric method allows the simultaneous identification and quantification of arachidonic acid metabolites in one single LC-MS/MS run.
UR - http://www.scopus.com/inward/record.url?scp=73549116102&partnerID=8YFLogxK
U2 - 10.1515/JLM.2009.057
DO - 10.1515/JLM.2009.057
M3 - Journal articles
AN - SCOPUS:73549116102
SN - 0342-3026
VL - 33
SP - 341
EP - 348
JO - LaboratoriumsMedizin
JF - LaboratoriumsMedizin
IS - 6
ER -