Serum CD5L as potential biomarker of thyroid hormone status during pregnancy

Sabrina Asaad; Thilo Samson Chillon; Dorota Filipowicz; Britta Wilms; Frank Strenge; Ewelina Szczepanek-Parulska; Waldemar B Minich; Sebastian M Meyhöfer; Jens U Marquardt; Jens Mittag; Henrik Oster; Marek Ruchala; Lutz Schomburg

doi:10.1002/biof.2123

Serum CD5L as potential biomarker of thyroid hormone status during pregnancy

Sabrina Asaad, Thilo Samson Chillon, Dorota Filipowicz, Britta Wilms, Frank Strenge, Ewelina Szczepanek-Parulska, Waldemar B Minich, Sebastian M Meyhöfer, Jens U Marquardt, Jens Mittag, Henrik Oster, Marek Ruchala, Lutz Schomburg

Poznan University of Medical Sciences

2 Citations (Scopus)

Abstract

The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by TH receptor beta, whereas many peripheral organs employ the alpha receptor. Serum cluster of differentiation 5-like molecule (CD5L) is a liver-derived protein under control of both TH receptor isoforms. However, clinical data on its relation to TH status are sparse. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed. This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy. A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper, and selenium concentrations. The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper. The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease.

Original language	English
Article number	e2123
Journal	BioFactors
Volume	51
Issue number	1
Pages (from-to)	e2123
ISSN	0951-6433
DOIs	https://doi.org/10.1002/biof.2123
Publication status	Published - 2024

Funding

Funders	Funder number
Deutsche Forschungsgemeinschaft	CRC/TR 296
Narodowe Centrum Nauki	2019/33/N/NZ5/02303

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

DFG Research Classification Scheme

2.22-17 Endocrinology, Diabetology, Metabolism
2.22-21 Gynaecology and Obstetrics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/biof.2123

Cite this

@article{f5cb7e28f1014d8b855a5d1036b1e315,

title = "Serum CD5L as potential biomarker of thyroid hormone status during pregnancy",

abstract = "The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by TH receptor beta, whereas many peripheral organs employ the alpha receptor. Serum cluster of differentiation 5-like molecule (CD5L) is a liver-derived protein under control of both TH receptor isoforms. However, clinical data on its relation to TH status are sparse. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed. This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy. A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper, and selenium concentrations. The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper. The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease.",

author = "Sabrina Asaad and Chillon, \{Thilo Samson\} and Dorota Filipowicz and Britta Wilms and Frank Strenge and Ewelina Szczepanek-Parulska and Minich, \{Waldemar B\} and Meyh{\"o}fer, \{Sebastian M\} and Marquardt, \{Jens U\} and Jens Mittag and Henrik Oster and Marek Ruchala and Lutz Schomburg",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). BioFactors published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology.",

year = "2024",

doi = "10.1002/biof.2123",

language = "English",

volume = "51",

pages = "e2123",

journal = "BioFactors",

issn = "0951-6433",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Serum CD5L as potential biomarker of thyroid hormone status during pregnancy

AU - Asaad, Sabrina

AU - Chillon, Thilo Samson

AU - Filipowicz, Dorota

AU - Wilms, Britta

AU - Strenge, Frank

AU - Szczepanek-Parulska, Ewelina

AU - Minich, Waldemar B

AU - Meyhöfer, Sebastian M

AU - Marquardt, Jens U

AU - Mittag, Jens

AU - Oster, Henrik

AU - Ruchala, Marek

AU - Schomburg, Lutz

PY - 2024

Y1 - 2024

N2 - The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by TH receptor beta, whereas many peripheral organs employ the alpha receptor. Serum cluster of differentiation 5-like molecule (CD5L) is a liver-derived protein under control of both TH receptor isoforms. However, clinical data on its relation to TH status are sparse. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed. This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy. A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper, and selenium concentrations. The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper. The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease.

AB - The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by TH receptor beta, whereas many peripheral organs employ the alpha receptor. Serum cluster of differentiation 5-like molecule (CD5L) is a liver-derived protein under control of both TH receptor isoforms. However, clinical data on its relation to TH status are sparse. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed. This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy. A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper, and selenium concentrations. The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper. The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease.

UR - https://www.scopus.com/pages/publications/85205494191

U2 - 10.1002/biof.2123

DO - 10.1002/biof.2123

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ER -

Serum CD5L as potential biomarker of thyroid hormone status during pregnancy

Abstract

Funding

Research Areas and Centers

DFG Research Classification Scheme

UN SDGs

Access to Document

Other files and links

Fingerprint

CRC/TRR 296 LocoTact: Local control of TH action

Cite this

Serum CD5L as potential biomarker of thyroid hormone status during pregnancy

Abstract

Funding

Research Areas and Centers

DFG Research Classification Scheme

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

CRC/TRR 296 LocoTact: Local control of TH action

Cite this