Sequential production of IL-2, IFN-γ and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes

Mario Assenmacher, Max Löhning, Alexander Scheffold, Rudolf A. Manz, Jürgen Schmitz, Andreas Radbruch

90 Citations (Scopus)


Upon primary activation, T helper (Th) cell populations express different cytokines transiently and with different kinetics. Stimulation of naive murine splenic Th cells with the bacterial superantigen Staphylococcus aureus enterotoxin B (SEB) in vitro results in expression of IL-2, IFN-γ and IL-10 with fast, intermediate and slow kinetics, respectively. This first report of a functional analysis of cells separated alive according to cytokine expression shows that these cytokines are not produced by different Th cell subpopulations, but can be expressed sequentially by individual Th cells. Th cells, activated with SEB for 1 day and isolated according to expression of IL-2, using the cellular affinity matrix technology, upon continued stimulation with SEB later secrete most of the IFN-γ and IL-10. Likewise, after 2 days of SEB culture, cells expressing IFN-γ, separated according to specific surface-associated IFN-γ as detected by magnetofluorescent liposomes, 1 day later secrete IL-10. Thus, individual Th1 cells can contribute to the control of their own IFN-γ expression by sequential expression of first IL-2, supporting their proliferation, and later IL-10, down-regulating the production of IFN-γ-inducing monokines and limiting the pro-inflammatory effects of IFN-γ.

Original languageEnglish
JournalEuropean Journal of Immunology
Issue number5
Pages (from-to)1534-1543
Number of pages10
Publication statusPublished - 05.1998

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


Dive into the research topics of 'Sequential production of IL-2, IFN-γ and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes'. Together they form a unique fingerprint.

Cite this