Sensory neurons respond to hypoxia with NO production associated with mitochondria

Michael Henrich; Karin Hoffmann; Peter König; Marco Gruß; Tamara Fischbach; Axel Gödecke; Gunter Hempelmann; Wolfgang Kummer

doi:10.1006/mcne.2002.1111

Sensory neurons respond to hypoxia with NO production associated with mitochondria

Michael Henrich^*, Karin Hoffmann, Peter König, Marco Gruß, Tamara Fischbach, Axel Gödecke, Gunter Hempelmann, Wolfgang Kummer

^*Corresponding author for this work

Institute of Anatomy

49 Citations (Scopus)

Abstract

Oxygen is pivotal for mammalian cell function, and recent studies suggest an 3involvement of NO in cellular adaptation to low oxygen supply. Here, we report that endothelial NO-synthase is ubiquitously expressed in rat and mice sensory neurons, and is targeted to juxtamitochondrial compartments of the ER. There it is activated in response to hypoxia while generation of reactive oxygen species remains unaltered. Developing a technique for ultrastructural localization of an NO-sensitive indicator allowed to identify the inner mitochondrial membrane as the target of NO under hypoxia. The demonstrated hypoxic stimulation of endothelial NOS in sensory neurons shall contribute to resistance against hypoxia, since NO promotes cellular survival by interfering with mitochondrial function.

Original language	English
Journal	Molecular and Cellular Neuroscience
Volume	20
Issue number	2
Pages (from-to)	307-322
Number of pages	16
ISSN	1044-7431
DOIs	https://doi.org/10.1006/mcne.2002.1111
Publication status	Published - 2002

Funding

We thank Andreas Scholz, Giessen, for providing samples of neuronal cytoplasm collected during intracellular recording experiments, Silke Wiegand, Martin Bodenbenner, and Gerhard Kripp for skillful technical assistance and Karola Michael for figure layout. This study was supported by the DFG (SFB 547, Project C1; GK 534).

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10.1006/mcne.2002.1111

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title = "Sensory neurons respond to hypoxia with NO production associated with mitochondria",

abstract = "Oxygen is pivotal for mammalian cell function, and recent studies suggest an 3involvement of NO in cellular adaptation to low oxygen supply. Here, we report that endothelial NO-synthase is ubiquitously expressed in rat and mice sensory neurons, and is targeted to juxtamitochondrial compartments of the ER. There it is activated in response to hypoxia while generation of reactive oxygen species remains unaltered. Developing a technique for ultrastructural localization of an NO-sensitive indicator allowed to identify the inner mitochondrial membrane as the target of NO under hypoxia. The demonstrated hypoxic stimulation of endothelial NOS in sensory neurons shall contribute to resistance against hypoxia, since NO promotes cellular survival by interfering with mitochondrial function.",

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journal = "Molecular and Cellular Neuroscience",

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T1 - Sensory neurons respond to hypoxia with NO production associated with mitochondria

AU - Henrich, Michael

AU - Hoffmann, Karin

AU - König, Peter

AU - Gruß, Marco

AU - Fischbach, Tamara

AU - Gödecke, Axel

AU - Hempelmann, Gunter

AU - Kummer, Wolfgang

N1 - Funding Information: We thank Andreas Scholz, Giessen, for providing samples of neuronal cytoplasm collected during intracellular recording experiments, Silke Wiegand, Martin Bodenbenner, and Gerhard Kripp for skillful technical assistance and Karola Michael for figure layout. This study was supported by the DFG (SFB 547, Project C1; GK 534). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - Oxygen is pivotal for mammalian cell function, and recent studies suggest an 3involvement of NO in cellular adaptation to low oxygen supply. Here, we report that endothelial NO-synthase is ubiquitously expressed in rat and mice sensory neurons, and is targeted to juxtamitochondrial compartments of the ER. There it is activated in response to hypoxia while generation of reactive oxygen species remains unaltered. Developing a technique for ultrastructural localization of an NO-sensitive indicator allowed to identify the inner mitochondrial membrane as the target of NO under hypoxia. The demonstrated hypoxic stimulation of endothelial NOS in sensory neurons shall contribute to resistance against hypoxia, since NO promotes cellular survival by interfering with mitochondrial function.

AB - Oxygen is pivotal for mammalian cell function, and recent studies suggest an 3involvement of NO in cellular adaptation to low oxygen supply. Here, we report that endothelial NO-synthase is ubiquitously expressed in rat and mice sensory neurons, and is targeted to juxtamitochondrial compartments of the ER. There it is activated in response to hypoxia while generation of reactive oxygen species remains unaltered. Developing a technique for ultrastructural localization of an NO-sensitive indicator allowed to identify the inner mitochondrial membrane as the target of NO under hypoxia. The demonstrated hypoxic stimulation of endothelial NOS in sensory neurons shall contribute to resistance against hypoxia, since NO promotes cellular survival by interfering with mitochondrial function.

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SP - 307

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Sensory neurons respond to hypoxia with NO production associated with mitochondria

Abstract

Funding

UN SDGs

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